Cloned (Comment) | Organism |
---|---|
overexpression with His-tag | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | both full-length enzyme and the isolated EAL domain hydrolyze cyclic diguanylate. Hydrolysis of 5-phosphoguanylyl(3-5)guanosine to guanosine monophosphate by isolated domain EAL is very poor | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | strong | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
cyclic di-3',5'-guanylate | pH 9.4 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 10% of the activity with Mg2+, Mn2+ in presence of Co2+ | Escherichia coli | |
Mg2+ | or Mn2+, absolutely required, with Mn2+ being slightly more efficient | Escherichia coli | |
Mn2+ | or Mg2+, absolutely required, with Mn2+ being slightly more efficient | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
isoform YahA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged protein | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclic di-3',5'-guanylate + H2O | specific substrate | Escherichia coli | 5'-phosphoguanylyl(3'-5')guanosine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
YahA | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.4 | - |
- |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
10 | - |
slight decrease in activity when raising pH value from 9.4 to 10 | Escherichia coli |