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Literature summary for 3.1.4.4 extracted from

  • Liu, Y.; Zhang, T.; Qiao, J.; Liu, X.; Bo, J.; Wang, J.; Lu, F.
    High-yield phosphatidylserine production via yeast surface display of phospholipase D from Streptomyces chromofuscus on Pichia pastoris (2014), J. Agric. Food Chem., 62, 5354-5360.
    View publication on PubMed
Search for more literature for 3.1.4.4

Cloned(Commentary)

Cloned (Comment) Organism
yeast surface display of phospholipase D from Streptomyces chromofuscus on Pichia pastoris strain GS115/pKFS-pldh using a Flo1p anchor attachment signal sequence Streptomyces chromofuscus

Protein Variants

Protein Variants Comment Organism
additional information high-yield phosphatidylserine production via yeast surface display of the enzyme in Pichia pastoris strain GS115/pKFS-pldh. Thermostability, acid stability and organic solvent tolerance of the dPLD are significantly enhanced compared with the secreted wild-type enzyme from Streptomyces chromofuscus Streptomyces chromofuscus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Streptomyces chromofuscus
-
-

Organism

Organism UniProt Comment Textmining
Streptomyces chromofuscus Q8KRK5 gene pld
-

Synonyms

Synonyms Comment Organism
PLD
-
 Streptomyces chromofuscus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 recombinant enzyme on Pichia pastoris cells Streptomyces chromofuscus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 65 recombinant enzyme on Pichia pastoris cells, stable within this range Streptomyces chromofuscus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 recombinant enzyme on Pichia pastoris cells Streptomyces chromofuscus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 11 recombinant enzyme on Pichia pastoris cells, stable within this range Streptomyces chromofuscus