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Literature summary for 3.1.4.4 extracted from
- Kinetic study of sunflower phospholipase Dalpha: Interactions with micellar substrate, detergents and metals (2011), Plant Physiol. Biochem., 49, 752-757.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | interacts with the SDS contained in the mixed micelles thus leading to an aggregated form of the substrate which is more easily hydrolyzed by PLDalpha | Helianthus annuus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Helianthus annuus | purified PLDalpha is inactive in vitro on bilamellar substrates. It is fully active on mixed micelles made with phospholipids and a mixture of Triton-X100 and SDS at equal concentrations. Ca2+ interacts with the SDS contained in the mixed micelles thus leading to an aggregated form of the substrate which is more easily hydrolyzed by PLDalpha | ? | - |
? | |
| phosphatidylcholine + H2O | Helianthus annuus | - |
choline + phosphatidate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helianthus annuus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native PLDalpha 25fold from six-day post-germinated seeds | Helianthus annuus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Helianthus annuus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 60 | - |
purified native enzyme, pH and temperature not specified in the publication | Helianthus annuus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | purified PLDalpha is inactive in vitro on bilamellar substrates. It is fully active on mixed micelles made with phospholipids and a mixture of Triton-X100 and SDS at equal concentrations. Ca2+ interacts with the SDS contained in the mixed micelles thus leading to an aggregated form of the substrate which is more easily hydrolyzed by PLDalpha | Helianthus annuus | ? | - |
? | |
| phosphatidylcholine + H2O | - |
Helianthus annuus | choline + phosphatidate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| phospholipase Dalpha | - |
Helianthus annuus |
| PLDalpha | - |
Helianthus annuus |
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Release 2023.1 (January 2023)
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