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Literature summary for 3.1.4.4 extracted from

  • Sun, Y.; Fang, Y.; Yoon, M.S.; Zhang, C.; Roccio, M.; Zwartkruis, F.J.; Armstrong, M.; Brown, H.A.; Chen, J.
    Phospholipase D1 is an effector of Rheb in the mTOR pathway (2008), Proc. Natl. Acad. Sci. USA, 105, 8286-8291.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.4

Activating Compound

Activating Compound Comment Organism Structure
additional information the overexpression of tuberous sclerosis complex 2, TSC2, suppresses PLD1 activation, whereas the knockdown or deletion of TSC2 leads to elevated basal activity of PLD Homo sapiens
Rheb Rheb binds and activates PLD1 in vitro in a GTP-dependent manner. Overexpression of the small GTPase Rheb activates PLD1 in cells by about 2.5fold in the absence of mitogenic stimulation, and the knockdown of Rheb impairs serum stimulation of PLD activation, overview Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
coexpression of HA-tagged PLD1 and various epitope-tagged GTPases in HEK-293 cells for interaction analysis, overview Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information knockdown of isozyme PLD2 by two independent shRNAs has no effect on Rheb activation of S6K1 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1-butanol
-
 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Homo sapiens
Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphatidylcholine + H2O Homo sapiens
-
 choline + phosphatidic acid
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell isozymes PLD1 and PLD2 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylcholine + H2O
-
 Homo sapiens choline + phosphatidic acid
-
 ?

Synonyms

Synonyms Comment Organism
phospholipase D1
-
 Homo sapiens
PLD
-
 Homo sapiens
PLD1
-
 Homo sapiens
PLD2
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

General Information

General Information Comment Organism
physiological function phospholipase D and its product phosphatidic acid are upstream regulators of the mitogenic mTOR signaling in both mitogenesis and the mechanical stimulation of skeletal muscle growth. Isozyme PLD1, but not PLD2, is required for Rheb activation of the mTOR pathway and for Rheb activation of S6K1, PLD1 is a bona fide effector for Rheb Homo sapiens