Protein Variants | Comment | Organism |
---|---|---|
additional information | antisense-mediated knockdown of tobacco pollen PLDs results in inhibition of pollen tube growth | Nicotiana tabacum |
N232A | site-directed mutagenesis of isozyme PLDbeta1, leads to reduced actin and G-protein binding | Nicotiana tabacum |
N323A/T382A | site-directed mutagenesis of isozyme PLDbeta1, leads to reduced actin and G-protein binding | Nicotiana tabacum |
T382A | site-directed mutagenesis of isozyme PLDbeta1, leads to reduced actin and G-protein binding | Nicotiana tabacum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by tert-butanol; no inhibition by tert-butanol; no inhibition by tert-butanol | Nicotiana tabacum | |
n-butanol | PLD inhibition disrupts the actin cytoskeleton in tobacco pollen tubes; PLD inhibition disrupts the actin cytoskeleton in tobacco pollen tubes; PLD inhibition disrupts the actin cytoskeleton in tobacco pollen tubes | Nicotiana tabacum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
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Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Nicotiana tabacum | ability of PLD-generated phosphatidic acid to control actin polymerization and the reciprocal ability of actin to specifically modulate PIP2-dependent PLD, PLDbeta, activity through direct interaction | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | D7P5D9 | cv. Samsun and Bright Yellow 2, different isozymes | - |
Nicotiana tabacum | D7P5E0 | cv. Samsun and Bright Yellow 2, isozyme PLDbeta1 | - |
Nicotiana tabacum | D7P5E1 | cv. Samsun and Bright Yellow 2, isozyme PLDdelta | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | from cv. Bright Yellow 2 | Nicotiana tabacum | - |
additional information | semi-quantitative PCR analysis of PLD expression and tissue distribvution | Nicotiana tabacum | - |
pollen | from cv. Samsun | Nicotiana tabacum | - |
pollen tube | - |
Nicotiana tabacum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | ability of PLD-generated phosphatidic acid to control actin polymerization and the reciprocal ability of actin to specifically modulate PIP2-dependent PLD, PLDbeta, activity through direct interaction | Nicotiana tabacum | ? | - |
? | |
additional information | enhanced binding of isozyme PLDbeta1 to actin is mediated by amino acid residues Asn323 and Thr382 | Nicotiana tabacum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PIP2-independent PLD | - |
Nicotiana tabacum |
PLD | - |
Nicotiana tabacum |
PLDalpha | - |
Nicotiana tabacum |
PLDalpha3 | - |
Nicotiana tabacum |
PLDbeta | - |
Nicotiana tabacum |
PLDbeta1 | - |
Nicotiana tabacum |
PLDdelta | - |
Nicotiana tabacum |
General Information | Comment | Organism |
---|---|---|
malfunction | PLD inhibition disrupts the actin cytoskeleton in tobacco pollen tubes, with severe disorganization of actin, both in the apex and in the shank | Nicotiana tabacum |
physiological function | PLD activity controls the structure of the actin cytoskeleton in tobacco pollen tubes, actin forms affect differently the activity of distinct tobacco C2 PLDs, overview | Nicotiana tabacum |