Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.4.4 extracted from

  • Lee, J.S.; Bat-Ochir, M.; Demirev, A.V.; Nam, D.H.
    Molecular cloning of the phospholipase D gene from Streptomyces sp. YU100 and its expression in Escherichia coli (2009), J. Microbiol., 47, 116-122.
    View publication on PubMed
Search for more literature for 3.1.4.4

Cloned(Commentary)

Cloned (Comment) Organism
recombinantly expressed in Escherchia coli as a His-tagged fusion protein Streptomyces sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 x * 54000, calculated from cDNA Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp. B0FZD9
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Streptomyces sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 wild-type protein shows nearly the same enzymatic activity as the recombinant protein produced in Escherichia coli Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylcholine + H2O
-
 Streptomyces sp. phosphatidate + choline
-
 ?
phosphatidylcholine + L-serine
-
 Streptomyces sp. phosphatidyl-L-serine + choline
-
 ?

Subunits

Subunits Comment Organism
? x * 54000, calculated from cDNA Streptomyces sp.

Synonyms

Synonyms Comment Organism
phospholipase D
-
 Streptomyces sp.
PLD
-
 Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 transphosphatidylation reaction at Streptomyces sp.
37
-
 hydrolytic reaction at Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Streptomyces sp.