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Literature summary for 3.1.4.4 extracted from

  • Lerchner, A.; Mansfeld, J.; Kuppe, K.; Ulbrich-Hofmann, R.
    Probing conserved amino acids in phospholipase D (Brassica oleracea var. capitata) for their importance in hydrolysis and transphosphatidylation activity (2006), Protein Eng. Des. Sel., 19, 443-452.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
C310S significant increase in total and transphosphatidylation activity Brassica oleracea
C625S significant increase in total and transphosphatidylation activity Brassica oleracea
additional information creation of 35 enzyme variants bearing mutations in the two HKD motifs, the C-terminus, or the eight cysteine resiudes. Analysis of transphosphatidylation activity and hydrolytic activity of mutants. All positions tested prove to be very sensitive towards amino acid exchanges with respect to hydrolytic activity in the absence of glycerol as well as to the ratio of hydrolytic and transphosphatidylation activities in the presence of glycerol Brassica oleracea

Organism

Organism UniProt Comment Textmining
Brassica oleracea
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isoform PLD2
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Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Brassica oleracea
-