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Literature summary for 3.1.4.4 extracted from

  • Simkhada, J.R.; Cho, S.S.; Lee, H.J.; Yoo, J.C.
    Purification and biochemical properties of phospholipase D (PLD57) produced by Streptomyces sp. CS-57 (2007), Arch. Pharm. Res., 30, 1302-1308.
    View publication on PubMed
Search for more literature for 3.1.4.4

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 0.375%, 75% increase in activity Streptomyces sp.

Inhibitors

Inhibitors Comment Organism Structure
deoxycholic acid 0.45%, 26% residual activity Streptomyces sp.
FeSO4 2 mM, 78% of initial activity Streptomyces sp.
N-laurylsarcosine 0.45%, 0% residual activity Streptomyces sp.
polyoxyethylene-4-lauryl ether 0.45%, 21% residual activity Streptomyces sp.
sodium dodecylsulfate 0.45%, 3% residual activity Streptomyces sp.
Tween 20 0.45%, 3% residual activity Streptomyces sp.
Tween 80 0.45%, 5% residual activity Streptomyces sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Streptomyces sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
CoSO4 2 mM, 106% of initial activity Streptomyces sp.
EDTA 2 mM, 139% of initial activity Streptomyces sp.
MnSO4 2 mM, 116% of initial activity Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 x * 55000, SDS-PAGE Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-
Streptomyces sp. CS-57
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
30
-
 pH 8.0, 55°C Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information in presence of glycerol, the rate of hydrolysis of phosphatiylcholine and the rate of phosphytidylglycerol formation are almost identical Streptomyces sp. ?
-
 ?
additional information in presence of glycerol, the rate of hydrolysis of phosphatiylcholine and the rate of phosphytidylglycerol formation are almost identical Streptomyces sp. CS-57 ?
-
 ?
phosphatidylcholine + glycerol
-
 Streptomyces sp. phosphatidylglycerol + choline
-
 ?
phosphatidylcholine + glycerol
-
 Streptomyces sp. CS-57 phosphatidylglycerol + choline
-
 ?
phosphatidylcholine + H2O
-
 Streptomyces sp. 1,2-diacylglycerophosphate + choline
-
 ?
phosphatidylcholine + H2O
-
 Streptomyces sp. CS-57 1,2-diacylglycerophosphate + choline
-
 ?
phosphatidylcholine + inositol
-
 Streptomyces sp. phosphatidylinositol + choline
-
 ?
phosphatidylcholine + inositol
-
 Streptomyces sp. CS-57 phosphatidylinositol + choline
-
 ?
phosphatidylcholine + L-serine
-
 Streptomyces sp. phosphatidylserine + choline
-
 ?
phosphatidylcholine + L-serine
-
 Streptomyces sp. CS-57 phosphatidylserine + choline
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 transphosphatidylation activity Streptomyces sp.
45
-
 hydrolytic activity Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 2 h, no loss of activity Streptomyces sp.
55
-
 20 min, complete loss of activity Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 both hydrolytic and transphosphatidylation activity Streptomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8
-
 Streptomyces sp.