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Literature summary for 3.1.4.4 extracted from
- Recognition of phospholipids in Streptomyces phospholipase D (2005), J. Biol. Chem., 280, 26143-26151.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of chimeras between TH-2PLD and PLD from a distict Streptomyces sp. | Streptomyces septatus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km-values of chimeric enzymes | Streptomyces septatus | |
| 0.075 | - |
phosphatidyl-p-nitrophenol | pH 5.5, hydrolysis | Streptomyces septatus |  |
| 1.16 | - |
phosphatidyl-p-nitrophenol | pH 5.5, transphosphatidylation | Streptomyces septatus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces septatus | - |
recombinant enzyme | - |
| Streptomyces septatus TH-2 | - |
recombinant enzyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme expressed in Escherichia coli | Streptomyces septatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidyl-p-nitrophenol + H2O | the N-terminal HKD motif contains the catalytic nucleophile, which attacks the phosphatidyl group of the substrate | Streptomyces septatus | phosphatidate + p-nitrophenol | - |
? | |
| phosphatidyl-p-nitrophenol + H2O | the N-terminal HKD motif contains the catalytic nucleophile, which attacks the phosphatidyl group of the substrate | Streptomyces septatus TH-2 | phosphatidate + p-nitrophenol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TH-2PLD | - |
Streptomyces septatus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | turnover-numbers of chimeric enzymes | Streptomyces septatus | |
| 68.8 | - |
phosphatidyl-p-nitrophenol | pH 5.5, hydrolysis | Streptomyces septatus | |
| 174.8 | - |
phosphatidyl-p-nitrophenol | pH 5.5, transphosphatidylation | Streptomyces septatus | |
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Release 2023.1 (January 2023)
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