Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphatidylinositol 4,5-bisphosphate | the interaction of Ca2+ with PLDbetacat (PLD with a deleted regulatory C2 domain) increases the affinity of the protein for the activator phosphatidylinositol 4,5-bisphosphate. Ca2+ binding to the C2 domain stimulates phosphatidylcholine binding but inhibits phosphatidylinositol 4,5-bisphosphate | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
wild-type PLDbeta, PLDbetacat with a deleted C2 domain, expression in Escherichia coli | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
DELTA1-158 | PLD with a deleted regulatory C2 domain exhibits Ca2+-dependent activity, is much less active and requires a higher level of Ca2+ than the full-length enzyme PLDbeta | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | interacts with the catalytic regions of the enzyme. PLDbetacat (PLD with a deleted regulatory C2 domain) exhibits Ca2+-dependent activity, is much less active and requires a higher level of Ca2+ than the full-length enzyme PLDbeta. The interaction of Ca2+ with PLDbetacat (PLD with a deleted regulatory C2 domain) increases the affinity of the protein for the activator phosphatidylinositol 4,5-bisphosphate. Ca2+ binding to the C2 domain stimulates phosphatidylcholine binding but inhibits phosphatidylinositol 4,5-bisphosphate | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + H2O | - |
Arabidopsis thaliana | 1,2-diacylglycerophosphate + choline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
phospholipase Dbeta | - |
Arabidopsis thaliana |
PLDbeta | - |
Arabidopsis thaliana |