Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.4.4 extracted from

  • Sharma, S.; Gupta, M.N.
    Purification of phospholipase D from Dacus carota by three-phase partitioning and its characterization (2001), Protein Expr. Purif., 21, 310-316.
    View publication on PubMed
Search for more literature for 3.1.4.4

Activating Compound

Activating Compound Comment Organism Structure
sodium dodecylsulfate in molar ratio phosphatidylcholine to SDS 4.32:1 Daucus carota
Triton X-100 in molar ratio phosphatidylcholine to Triton X-100 2.5:1 Daucus carota

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
9.5
-
 p-Nitrophenylphosphorylcholine pH 7.0, 25°C Daucus carota

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ optimal at 30 mM Daucus carota

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 x * 60000, SDS-PAGE Daucus carota

Organism

Organism UniProt Comment Textmining
Daucus carota
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Daucus carota

Purification (Commentary)

Purification (Comment) Organism
-
 Daucus carota

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
933
-
 pH 7.0, 25°C Daucus carota

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenylphosphorylcholine + H2O
-
 Daucus carota ?
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Daucus carota

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30
-
 unstable above Daucus carota

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5
-
 Daucus carota

pH Range

pH Minimum pH Maximum Comment Organism
7 8
-
 Daucus carota