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Literature summary for 3.1.4.4 extracted from

  • Shimbo, K.; Iwasaki,Y.; Yamane, T.; Ina,K.
    Purification and properties of phospholipase D from Streptomyces antibioticus (1993), Biosci. Biotechnol. Biochem., 57, 1946-1948.
No PubMed abstract available
Search for more literature for 3.1.4.4

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
 Streptomyces antibioticus
dithiothreitol
-
 Streptomyces antibioticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
 chromatofocusing, SDS-PAGE Streptomyces antibioticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phospholipid + alcohol Streptomyces antibioticus transphosphaditylation phospholipid + alcohol
-
 ?
phospholipid + H2O Streptomyces antibioticus phosphoric ester hydrolysis phosphatidic acid + alcohol
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces antibioticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Streptomyces antibioticus

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant
-
 Streptomyces antibioticus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1437
-
-
 Streptomyces antibioticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lysophosphatidylcholine + H2O
-
 Streptomyces antibioticus monoacylglycerophosphate + choline
-
 ?
phosphatidylcholine + D-arabinose
-
 Streptomyces antibioticus phosphatidylarabinose + choline
-
 ?
phosphatidylcholine + D-fructose
-
 Streptomyces antibioticus phosphatidylfructose + choline
-
 ?
phosphatidylcholine + D-galactose
-
 Streptomyces antibioticus phosphatidylgalactose + choline
-
 ?
phosphatidylcholine + D-glucose
-
 Streptomyces antibioticus phosphatidylglucose + choline
-
 ?
phosphatidylcholine + D-mannose
-
 Streptomyces antibioticus phosphatidylmannose + choline
-
 ?
phosphatidylcholine + D-xylose
-
 Streptomyces antibioticus phosphatidylxylose + choline
-
 ?
phosphatidylcholine + diethyleneglycol
-
 Streptomyces antibioticus phosphatidyldiethyleneglycol + choline
-
 ?
phosphatidylcholine + diethyleneglycol monomethyl ester
-
 Streptomyces antibioticus diethyleneglycol dimethyl phosphatidic acid + choline
-
 ?
phosphatidylcholine + ethyleneglycol
-
 Streptomyces antibioticus phosphatidylethyleneglycol + choline
-
 ?
phosphatidylcholine + ethyleneglycol monomethyl ester
-
 Streptomyces antibioticus ethyleneglycol monomethyl phosphatidic acid + choline
-
 ?
phosphatidylcholine + H2O
-
 Streptomyces antibioticus 1,2-diacylglycerophosphate + choline
-
 ?
phosphatidylcholine + L-sorbose
-
 Streptomyces antibioticus phosphatidylsorbose + choline
-
 ?
phosphatidylcholine + triethyleneglycol
-
 Streptomyces antibioticus phosphatidyltriethyleneglycol + choline
-
 ?
phosphatidylcholine + triethyleneglycol monomethyl ester
-
 Streptomyces antibioticus triethyleneglycol trimethyl phosphatidic acid + choline
-
 ?
phosphatidylethanolamine + H2O
-
 Streptomyces antibioticus 1,2-diacylglycerophosphate + ethanolamine
-
 r
phospholipid + alcohol transphosphaditylation Streptomyces antibioticus phospholipid + alcohol
-
 ?
phospholipid + H2O phosphoric ester hydrolysis Streptomyces antibioticus phosphatidic acid + alcohol
-
 ?
sphingomyelin + H2O
-
 Streptomyces antibioticus N-acylsphingosylphosphate + choline
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 70
-
 Streptomyces antibioticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Streptomyces antibioticus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8 stable after 2 h at 37°C Streptomyces antibioticus
5.5
-
 50% loss of activity after 30 min at 70°C Streptomyces antibioticus