Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.4.37 extracted from

  • Keppetipola, N.; Shuman, S.
    Characterization of the 2,3 cyclic phosphodiesterase activities of Clostridium thermocellum polynucleotide kinase-phosphatase and bacteriophage lambda phosphatase (2007), Nucleic Acids Res., 35, 7721-7732.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.37

Protein Variants

Protein Variants Comment Organism
D233A catalytic activity is neligible Acetivibrio thermocellus
D233E decrease in catalytic efficiency by factor 9 Acetivibrio thermocellus
D233N catalytic activity is neligible Acetivibrio thermocellus
D236A increase in enzyme affinity for substrate Acetivibrio thermocellus
D236E kinetics similar to wild-type Acetivibrio thermocellus
D236N increase in enzyme affinity for substrate Acetivibrio thermocellus
D392A decrease of both kcat and Km value Acetivibrio thermocellus
D392E 2fold increase in kcat value without affecting Km value Acetivibrio thermocellus
D392N 2fold increase in kcat value without affecting Km value Acetivibrio thermocellus
H189A slight increase of Km value with 6fold decrease in kcat value Acetivibrio thermocellus
H189D 4fold increase in kcat value without affecting Km value Acetivibrio thermocellus
H189E 2fold increase in kcat value without affecting Km value Acetivibrio thermocellus
H323A kinetics similar to wild-type Acetivibrio thermocellus
H323Q kinetics similar to wild-type Acetivibrio thermocellus
H376D 60% increase in catalytic efficiency Acetivibrio thermocellus
H376N twofold increase in catalytic efficiency Acetivibrio thermocellus
R237A 4fold increase in Km value Acetivibrio thermocellus
R237K 6fold increase in Km value Acetivibrio thermocellus
R237Q 3fold increase in Km value Acetivibrio thermocellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.9
-
 cyclic 2',3'-AMP mutant H376D, pH 7.5, 45°C Acetivibrio thermocellus
4.7
-
 cyclic 2',3'-AMP mutant H376N, pH 7.5, 45°C Acetivibrio thermocellus
6.6
-
 cyclic 2',3'-AMP mutant D236N, pH 7.5, 45°C Acetivibrio thermocellus
7.8
-
 cyclic 2',3'-AMP mutant D236A, pH 7.5, 45°C Acetivibrio thermocellus
18
-
 cyclic 2',3'-AMP mutant D236E, pH 7.5, 45°C Acetivibrio thermocellus
18
-
 cyclic 2',3'-AMP mutant D392E, pH 7.5, 45°C Acetivibrio thermocellus
18
-
 cyclic 2',3'-AMP wild-type, pH 7.5, 45°C Acetivibrio thermocellus
20
-
 cyclic 2',3'-AMP mutant H189D, pH 7.5, 45°C Acetivibrio thermocellus
24
-
 cyclic 2',3'-AMP mutant H323Q, pH 7.5, 45°C Acetivibrio thermocellus
28
-
 cyclic 2',3'-AMP mutant H189E, pH 7.5, 45°C Acetivibrio thermocellus
29
-
 cyclic 2',3'-AMP mutant H189A, pH 7.5, 45°C Acetivibrio thermocellus
31
-
 cyclic 2',3'-AMP mutant D392N, pH 7.5, 45°C Acetivibrio thermocellus
32
-
 cyclic 2',3'-AMP mutant H323A, pH 7.5, 45°C Acetivibrio thermocellus
60
-
 cyclic 2',3'-AMP mutant R237Q, pH 7.5, 45°C Acetivibrio thermocellus
62
-
 cyclic 2',3'-AMP mutant D233E, pH 7.5, 45°C Acetivibrio thermocellus
77
-
 cyclic 2',3'-AMP mutant R237A, pH 7.5, 45°C Acetivibrio thermocellus
100
-
 cyclic 2',3'-AMP mutant D392A, pH 7.5, 45°C Acetivibrio thermocellus
100
-
 cyclic 2',3'-AMP mutant R237K, pH 7.5, 45°C Acetivibrio thermocellus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
 polynucleotide kinase-phosphatase
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclic 2',3'-AMP + H2O
-
 Acetivibrio thermocellus 2'-AMP
-
 ?

Synonyms

Synonyms Comment Organism
PNKP
-
 Acetivibrio thermocellus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.45
-
 cyclic 2',3'-AMP mutant H189A, pH 7.5, 45°C Acetivibrio thermocellus
1.8
-
 cyclic 2',3'-AMP mutant D392A, pH 7.5, 45°C Acetivibrio thermocellus
3.2
-
 cyclic 2',3'-AMP mutant H376D, pH 7.5, 45°C Acetivibrio thermocellus
3.25
-
 cyclic 2',3'-AMP mutant D233E, pH 7.5, 45°C Acetivibrio thermocellus
4.5
-
 cyclic 2',3'-AMP mutant D236A, pH 7.5, 45°C Acetivibrio thermocellus
5.1
-
 cyclic 2',3'-AMP mutant H376N, pH 7.5, 45°C Acetivibrio thermocellus
5.8
-
 cyclic 2',3'-AMP mutant D236N, pH 7.5, 45°C Acetivibrio thermocellus
6
-
 cyclic 2',3'-AMP mutant D236E, pH 7.5, 45°C Acetivibrio thermocellus
6.5
-
 cyclic 2',3'-AMP mutant H323Q, pH 7.5, 45°C Acetivibrio thermocellus
8.6
-
 cyclic 2',3'-AMP mutant R237A, pH 7.5, 45°C Acetivibrio thermocellus
8.9
-
 cyclic 2',3'-AMP wild-type, pH 7.5, 45°C Acetivibrio thermocellus
9.1
-
 cyclic 2',3'-AMP mutant H323A, pH 7.5, 45°C Acetivibrio thermocellus
15.9
-
 cyclic 2',3'-AMP mutant D392E, pH 7.5, 45°C Acetivibrio thermocellus
17.2
-
 cyclic 2',3'-AMP mutant H189E, pH 7.5, 45°C Acetivibrio thermocellus
18.7
-
 cyclic 2',3'-AMP mutant D392N, pH 7.5, 45°C Acetivibrio thermocellus
20.2
-
 cyclic 2',3'-AMP mutant R237Q, pH 7.5, 45°C Acetivibrio thermocellus
26.2
-
 cyclic 2',3'-AMP mutant R237K, pH 7.5, 45°C Acetivibrio thermocellus
34.3
-
 cyclic 2',3'-AMP mutant H189D, pH 7.5, 45°C Acetivibrio thermocellus