Protein Variants | Comment | Organism |
---|---|---|
D233A | catalytic activity is neligible | Acetivibrio thermocellus |
D233E | decrease in catalytic efficiency by factor 9 | Acetivibrio thermocellus |
D233N | catalytic activity is neligible | Acetivibrio thermocellus |
D236A | increase in enzyme affinity for substrate | Acetivibrio thermocellus |
D236E | kinetics similar to wild-type | Acetivibrio thermocellus |
D236N | increase in enzyme affinity for substrate | Acetivibrio thermocellus |
D392A | decrease of both kcat and Km value | Acetivibrio thermocellus |
D392E | 2fold increase in kcat value without affecting Km value | Acetivibrio thermocellus |
D392N | 2fold increase in kcat value without affecting Km value | Acetivibrio thermocellus |
H189A | slight increase of Km value with 6fold decrease in kcat value | Acetivibrio thermocellus |
H189D | 4fold increase in kcat value without affecting Km value | Acetivibrio thermocellus |
H189E | 2fold increase in kcat value without affecting Km value | Acetivibrio thermocellus |
H323A | kinetics similar to wild-type | Acetivibrio thermocellus |
H323Q | kinetics similar to wild-type | Acetivibrio thermocellus |
H376D | 60% increase in catalytic efficiency | Acetivibrio thermocellus |
H376N | twofold increase in catalytic efficiency | Acetivibrio thermocellus |
R237A | 4fold increase in Km value | Acetivibrio thermocellus |
R237K | 6fold increase in Km value | Acetivibrio thermocellus |
R237Q | 3fold increase in Km value | Acetivibrio thermocellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.9 | - |
cyclic 2',3'-AMP | mutant H376D, pH 7.5, 45°C | Acetivibrio thermocellus | |
4.7 | - |
cyclic 2',3'-AMP | mutant H376N, pH 7.5, 45°C | Acetivibrio thermocellus | |
6.6 | - |
cyclic 2',3'-AMP | mutant D236N, pH 7.5, 45°C | Acetivibrio thermocellus | |
7.8 | - |
cyclic 2',3'-AMP | mutant D236A, pH 7.5, 45°C | Acetivibrio thermocellus | |
18 | - |
cyclic 2',3'-AMP | mutant D236E, pH 7.5, 45°C | Acetivibrio thermocellus | |
18 | - |
cyclic 2',3'-AMP | mutant D392E, pH 7.5, 45°C | Acetivibrio thermocellus | |
18 | - |
cyclic 2',3'-AMP | wild-type, pH 7.5, 45°C | Acetivibrio thermocellus | |
20 | - |
cyclic 2',3'-AMP | mutant H189D, pH 7.5, 45°C | Acetivibrio thermocellus | |
24 | - |
cyclic 2',3'-AMP | mutant H323Q, pH 7.5, 45°C | Acetivibrio thermocellus | |
28 | - |
cyclic 2',3'-AMP | mutant H189E, pH 7.5, 45°C | Acetivibrio thermocellus | |
29 | - |
cyclic 2',3'-AMP | mutant H189A, pH 7.5, 45°C | Acetivibrio thermocellus | |
31 | - |
cyclic 2',3'-AMP | mutant D392N, pH 7.5, 45°C | Acetivibrio thermocellus | |
32 | - |
cyclic 2',3'-AMP | mutant H323A, pH 7.5, 45°C | Acetivibrio thermocellus | |
60 | - |
cyclic 2',3'-AMP | mutant R237Q, pH 7.5, 45°C | Acetivibrio thermocellus | |
62 | - |
cyclic 2',3'-AMP | mutant D233E, pH 7.5, 45°C | Acetivibrio thermocellus | |
77 | - |
cyclic 2',3'-AMP | mutant R237A, pH 7.5, 45°C | Acetivibrio thermocellus | |
100 | - |
cyclic 2',3'-AMP | mutant D392A, pH 7.5, 45°C | Acetivibrio thermocellus | |
100 | - |
cyclic 2',3'-AMP | mutant R237K, pH 7.5, 45°C | Acetivibrio thermocellus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required | Acetivibrio thermocellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | - |
polynucleotide kinase-phosphatase | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclic 2',3'-AMP + H2O | - |
Acetivibrio thermocellus | 2'-AMP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PNKP | - |
Acetivibrio thermocellus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.45 | - |
cyclic 2',3'-AMP | mutant H189A, pH 7.5, 45°C | Acetivibrio thermocellus | |
1.8 | - |
cyclic 2',3'-AMP | mutant D392A, pH 7.5, 45°C | Acetivibrio thermocellus | |
3.2 | - |
cyclic 2',3'-AMP | mutant H376D, pH 7.5, 45°C | Acetivibrio thermocellus | |
3.25 | - |
cyclic 2',3'-AMP | mutant D233E, pH 7.5, 45°C | Acetivibrio thermocellus | |
4.5 | - |
cyclic 2',3'-AMP | mutant D236A, pH 7.5, 45°C | Acetivibrio thermocellus | |
5.1 | - |
cyclic 2',3'-AMP | mutant H376N, pH 7.5, 45°C | Acetivibrio thermocellus | |
5.8 | - |
cyclic 2',3'-AMP | mutant D236N, pH 7.5, 45°C | Acetivibrio thermocellus | |
6 | - |
cyclic 2',3'-AMP | mutant D236E, pH 7.5, 45°C | Acetivibrio thermocellus | |
6.5 | - |
cyclic 2',3'-AMP | mutant H323Q, pH 7.5, 45°C | Acetivibrio thermocellus | |
8.6 | - |
cyclic 2',3'-AMP | mutant R237A, pH 7.5, 45°C | Acetivibrio thermocellus | |
8.9 | - |
cyclic 2',3'-AMP | wild-type, pH 7.5, 45°C | Acetivibrio thermocellus | |
9.1 | - |
cyclic 2',3'-AMP | mutant H323A, pH 7.5, 45°C | Acetivibrio thermocellus | |
15.9 | - |
cyclic 2',3'-AMP | mutant D392E, pH 7.5, 45°C | Acetivibrio thermocellus | |
17.2 | - |
cyclic 2',3'-AMP | mutant H189E, pH 7.5, 45°C | Acetivibrio thermocellus | |
18.7 | - |
cyclic 2',3'-AMP | mutant D392N, pH 7.5, 45°C | Acetivibrio thermocellus | |
20.2 | - |
cyclic 2',3'-AMP | mutant R237Q, pH 7.5, 45°C | Acetivibrio thermocellus | |
26.2 | - |
cyclic 2',3'-AMP | mutant R237K, pH 7.5, 45°C | Acetivibrio thermocellus | |
34.3 | - |
cyclic 2',3'-AMP | mutant H189D, pH 7.5, 45°C | Acetivibrio thermocellus |