Cloned (Comment) | Organism |
---|---|
full-length CNP1 and C-terminal deletion mutant, fused to glutathione S-transferase, expression in Escherichia coli JM83, fused to green fluorescent protein, expression in COS-7 cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | C-terminal deletion of 13 amino acids of CNP1 leads to an abnormal microtubule distribution in the cell | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-bound, microtubule-associated protein, membrane anchor for tubulin | Rattus norvegicus | 16020 | - |
microtubule | tubulin from brain, microtubule-associated protein, membrane anchor for tubulin, membrane-bound | Rattus norvegicus | 5874 | - |
additional information | subcellular distribution of CNP | Rattus norvegicus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | CNP acts as microtubule-associated protein in promoting microtubule assembly at low mole ratios, links tubulin to membranes and may regulate cytoplasmic microtubule distribution, membrane anchor for tubulin | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | CNP is phosphorylated in vivo by protein kinase C, phosphorylation of CNP1 interferes with its microtubule assembly-promoting activity | Rattus norvegicus |
side-chain modification | CNP is isoprenylated and palmitoylated | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
recombinant CNP1 and C-terminal deletion mutant | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | CNP is associated with tubulin from brain | Rattus norvegicus | - |
FRTL-5 cell | ATCC CRL 8305, thyroid cells, CNP is firmly associated with FRTL-5 cells | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2',3'-cAMP + H2O | - |
Rattus norvegicus | 2'-AMP | - |
? | |
additional information | CNP acts as microtubule-associated protein in promoting microtubule assembly at low mole ratios, links tubulin to membranes and may regulate cytoplasmic microtubule distribution, membrane anchor for tubulin | Rattus norvegicus | ? | - |
? | |
nucleoside 2',3'-cyclic phosphate + H2O | activity resides in the C-terminus | Rattus norvegicus | nucleoside 2'-phosphate | - |
? |