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Literature summary for 3.1.4.37 extracted from

  • Seki, T.; Fukuda, S.
    Purification and some properties of 2',3'-cyclic phosphodiesterase from the cell-free extract of Bacillus subtilis var. amyloliquefacus (1981), J. Gen. Appl. Microbiol., 27, 487-498.
No PubMed abstract available
Search for more literature for 3.1.4.37

Inhibitors

Inhibitors Comment Organism Structure
Co2+
-
 Bacillus subtilis
Cu2+
-
 Bacillus subtilis
EDTA
-
 Bacillus subtilis
Hg2+
-
 Bacillus subtilis
Zn2+
-
 Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.046
-
 3'-AMP
-
 Bacillus subtilis
0.16
-
 bis-p-nitrophenyl phosphate
-
 Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activation of activity against bis-p-nitrophenyl phosphate Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
 x * 74000, SDS-PAGE Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
 var. amyloliquefacus
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bacillus subtilis

Storage Stability

Storage Stability Organism
0-4°C, 5 mM Tris-HCl buffer, pH 7.6, stable for 2 months Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-AMP + H2O
-
 Bacillus subtilis ?
-
 ?
bis-p nitrophenyl phosphate + H2O
-
 Bacillus subtilis ?
-
 ?
additional information
-
 Bacillus subtilis ?
-
 ?

Subunits

Subunits Comment Organism
? x * 74000, SDS-PAGE Bacillus subtilis

Synonyms

Synonyms Comment Organism
2',3'-cyclic phosphodiesterase
-
 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 70 inactivation Bacillus subtilis
100
-
 80% loss of activity, and completely eliminated after 20 min, Co2+ protects against heat inactivation Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 activity against bis-p-nitrophenyl phosphate Bacillus subtilis
6.7
-
-
 Bacillus subtilis