Literature summary for 3.1.4.35 extracted from

Muradov, H.; Boyd, K.K.; Haeri, M.; Kerov, V.; Knox, B.E.; Artemyev, N.O.
Characterization of human cone phosphodiesterase-6 ectopically expressed in Xenopus laevis rods (2009), J. Biol. Chem., 284, 32662-32669.

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Cloned(Commentary)

Cloned (Comment)	Organism
ectopic expression of the enhanced green fluorescent protein fusion protein of human cone PDE6C in rods of transgenic Xenopus laevis	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
cone Pgamma subunit	PDE6C is potently inhibited by the recombinant cone and rod Pgamma-subunits with slight selectivity for the cone Pgamma	Homo sapiens
rod Pgamma subunit	PDE6C is potently inhibited by the recombinant cone and rod Pgamma-subunits with slight selectivity for the cone Pgamma	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.065	-	3',5'-cGMP	recombinant human cone PDE6C, pH and temperature not specified in the publication	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
125000	-	recombinant enhanced green fluorescent protein fusion protein of human cone PDE6C	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
immunoprecipitation with Dynabeads with Protein G	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3',5'-cGMP + H2O	PDE6 shows specificity for cGMP relative to cAMP	Homo sapiens	5'-GMP	-	?

Synonyms

Synonyms	Comment	Organism
PDE6AB	isoform	Homo sapiens
PDE6C	isoform	Homo sapiens
phosphodiesterase-6	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
4400	-	3',5'-cGMP	recombinant human cone PDE6C, pH and temperature not specified in the publication	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.078	-	cone Pgamma subunit	recombinant human cone PDE6C, pH and temperature not specified in the publication	Homo sapiens
0.105	-	rod Pgamma subunit	recombinant human cone PDE6AB, pH and temperature not specified in the publication	Homo sapiens
0.14	-	cone Pgamma subunit	recombinant human cone PDE6AB, pH and temperature not specified in the publication	Homo sapiens
0.155	-	rod Pgamma subunit	recombinant human cone PDE6C, pH and temperature not specified in the publication	Homo sapiens

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Release 2023.1 (January 2023)