Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.4.35 extracted from

  • Martinez, S.E.; Heikaus, C.C.; Klevit, R.E.; Beavo, J.A.
    The structure of the GAF A domain from phosphodiesterase 6C reveals determinants of cGMP binding, a conserved binding surface, and a large cGMP-dependent conformational change (2008), J. Biol. Chem., 283, 25913-25919.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.35

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Gallus gallus

Crystallization (Commentary)

Crystallization (Comment) Organism
GAF A domain of PDE6C, sitting drop vapour diffusion method, using 1.5-1.8 M ammonium sulfate, 0.1 M sodium acetate, pH 4.5-5.0 Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Gallus gallus PDE6 contains two regulatory GAF domains, of which one (GAF A) binds cGMP and regulates the activity of the PDE6 holoenzyme ?
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus gallus P52731
-
-

Purification (Commentary)

Purification (Comment) Organism
epoxy-Sepharose cGMP affinity resin column chromatography Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3',5'-cGMP + H2O
-
 Gallus gallus 5'-GMP
-
 ?
additional information PDE6 contains two regulatory GAF domains, of which one (GAF A) binds cGMP and regulates the activity of the PDE6 holoenzyme Gallus gallus ?
-
 ?

Subunits

Subunits Comment Organism
dimer full-length PDE6C, X-ray crystallography Gallus gallus
monomer PDE6C GAF A domain is monomeric and does not contain sufficient structural determinants to form a homodimer as found in full-length PDE6C, X-ray crystallography Gallus gallus

Synonyms

Synonyms Comment Organism
PDE6C
-
 Gallus gallus
phosphodiesterase 6C
-
 Gallus gallus