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Literature summary for 3.1.4.35 extracted from

  • Hurley, J.B.; Stryer, L.
    Purification and characterization of the gamma regulatory subunit of the cyclic GMP phosphodiesterase from retinal rod outer segments (1982), J. Biol. Chem., 257, 11094-11099.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
light activation Bos taurus
Trypsin
-
Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
Inhibitor protein gamma-subunit of enzyme Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.078
-
3',5'-cGMP trypsin activated enzyme Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
photoreceptor outer segment rod outer segment Bos taurus 1750
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
88000
-
88000, alpha-subunit, + 84000, beta-subunit, + 1100, gamma-subunit, inhibitory, SDS-PAGE Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
retina
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20 100 unactivated purified enzyme Bos taurus
1200 1500 trypsin-activated enzyme Bos taurus

Storage Stability

Storage Stability Organism
-20°C, 10 mM morpholinopropanesulfonic acid, 1.5 mM MgCl2, 0.1 mM phenylmethylsulfonyl fluoride, 1 mM dithiothreitol, 50% glycerol, pH 7.5, more than 6 months Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3',5'-cGMP + H2O
-
Bos taurus 5'-GMP
-
?

Subunits

Subunits Comment Organism
? 88000, alpha-subunit, + 84000, beta-subunit, + 1100, gamma-subunit, inhibitory, SDS-PAGE Bos taurus