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Literature summary for 3.1.4.3 extracted from

  • Seo, J.S.; Kim, M.S.; Lee, S.H.; Kim, K.H.; Lee, H.H.; Jeong, H.D.; Chung, J.K.
    Uronema marinum: identification and biochemical characterization of phosphatidylcholine-hydrolyzing phospholipase C (2005), Exp. Parasitol., 110, 22-29.
    View publication on PubMed
Search for more literature for 3.1.4.3

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol cPC-PLC Uronema marinum 5829
-
membrane mPC-PLC Uronema marinum 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 0.4 mM required for maximal activition, mPC-PLC Uronema marinum
Ca2+ 1.6 mM required for maximal activition, cPC-PLC Uronema marinum
Mg2+ 2.5 mM required for maximal activation, mPC-PLC Uronema marinum
Mg2+ 2.5 mM required for maximal activity, cPC-PLC Uronema marinum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Uronema marinum cPC-PLC may contribute to the parasite evasion against the host immune response ?
-
 ?
additional information Uronema marinum mPC-PLC may play a role in the intercellular multiplication of Uronema marinum ?
-
 ?

Organism

Organism UniProt Comment Textmining
Uronema marinum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, cPC-PLC Uronema marinum
partial, mPC-PLC Uronema marinum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dipalmitoylphosphatidylcholine + H2O
-
 Uronema marinum 1,2-dipalmitoylglycerol + phosphorylcholine
-
 ?
additional information cPC-PLC may contribute to the parasite evasion against the host immune response Uronema marinum ?
-
 ?
additional information mPC-PLC may play a role in the intercellular multiplication of Uronema marinum Uronema marinum ?
-
 ?
additional information no activity with phosphatidylinositol and phosphatidylethanolamine Uronema marinum ?
-
 ?

Synonyms

Synonyms Comment Organism
cPC-PLC
-
 Uronema marinum
mPC-PLC
-
 Uronema marinum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 cPC-PLC Uronema marinum
37
-
 mPC-PLC Uronema marinum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 37 25°C: about 55% of maximal activity, 37°C: optimum, cPC-PLC Uronema marinum
30 37 30°C: about 55% of maximal activity, 37°C: optimum, mPC-PLC Uronema marinum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 mPC-PLC Uronema marinum
7
-
 cPC-PLC Uronema marinum

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7 pH 4.5: about 60% of maximal activity, pH 7.0: about 60% of maximal activity, mPC-PLC Uronema marinum
6.5 7.5 pH 6.5: about 65% of maximal activity, pH 7.5: about 80% of maximal activity, cPC-PLC Uronema marinum