Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 2 mM, 40% inhibition | Pseudomonas sp. | |
CoCl2 | 2 mM, 90% inhibition | Pseudomonas sp. | |
CuSO3 | 2 mM, 96% inhibition | Pseudomonas sp. | |
dithiothreitol | 2 mM, 94% inhibition | Pseudomonas sp. | |
EDTA | 2 mM, 92% inhibition | Pseudomonas sp. | |
FeCl2 | 2 mM, 94% inhibition | Pseudomonas sp. | |
FeCl3 | 2 mM, 90% inhibition | Pseudomonas sp. | |
HgCl2 | 2 mM, 92% inhibition | Pseudomonas sp. | |
MnCl2 | 2 mM, 92% inhibition | Pseudomonas sp. | |
phenylmethylsulfonyl fluoride | 2 mM, 94% inhibition | Pseudomonas sp. | |
SDS | 2 mM, 90% inhibition | Pseudomonas sp. | |
ZnCl2 | 2 mM, 89% inhibition | Pseudomonas sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Pseudomonas sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme belongs to the group of metallophospholipases C | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. KS3.2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | phosphatidylethanolamine, sphingomyelin, phosphatidylinositol and phosphatidylserine are hydrolyzed at less than 1% of the activity with phosphatidylcholine | Pseudomonas sp. | ? | - |
? | |
additional information | phosphatidylethanolamine, sphingomyelin, phosphatidylinositol and phosphatidylserine are hydrolyzed at less than 1% of the activity with phosphatidylcholine | Pseudomonas sp. KS3.2 | ? | - |
? | |
phosphatidic acid + H2O | 9.04% of the activity with phosphatidylcholine | Pseudomonas sp. | ? | - |
? | |
phosphatidic acid + H2O | 9.04% of the activity with phosphatidylcholine | Pseudomonas sp. KS3.2 | ? | - |
? | |
phosphatidylcholine + H2O | - |
Pseudomonas sp. | 1,2-diacylglycerol + phosphorylcholine | - |
? | |
phosphatidylcholine + H2O | - |
Pseudomonas sp. KS3.2 | 1,2-diacylglycerol + phosphorylcholine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Pseudomonas sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 60 | 35°C: about 50% of maximal activity, 60°C: about 50% of maximal activity | Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
52 | - |
pH 7.2, 30 min, 50% loss of activity | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | pH 6.5: about 50% of maximal activity, pH 8.5: about 50% of maximal activity | Pseudomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
1 | 2 | 5°C, 3 h, about 55% loss of activity | Pseudomonas sp. |
6 | - |
5°C, 3 h, about 70% loss of activity | Pseudomonas sp. |
7 | - |
5°C, 3 h, about 35% loss of activity | Pseudomonas sp. |
8 | 9 | 5°C, 3 h, stable | Pseudomonas sp. |