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Literature summary for 3.1.4.3 extracted from

  • Hansen, S.; Hough, E.; Svensson, L.A.; Wong, Y.L.; Martin, S.F.
    Crystal structure of phospholipase C from Bacillus cereus complexed with a substrate analogue (1993), J. Mol. Biol., 234, 179-189.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
1.9 A resolution, R-factor of 15.7% Bacillus cereus
crystal structure of a complex between the enzyme and a competitive inhibitor. 1.9 A resolution, R-factor of 15.7% Bacillus cereus

Inhibitors

Inhibitors Comment Organism Structure
3(S),4-dihexanoylbutyl-1-phosphonycholine non-hydrolyzable, competitive inhibitor of bacterial PLC, analog of the natural phospholipid substrate, binds with its phosphonyl group to the three Zn-ions in the active site of the enzyme Bacillus cereus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.38
-
phosphatidylcholine water-soluble substrate Bacillus cereus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Bacillus cereus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ metalloenzyme Bacillus cereus
Zn2+ 3 Zn2+ ions in active site Bacillus cereus
Zn2+ in the active site of the enzyme Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylcholine + H2O
-
Bacillus cereus 1,2-diacylglycerol + choline phosphate
-
?
phosphatidylethanolamine + H2O
-
Bacillus cereus phosphorylethanolamine + diacylglycerol
-
?
phosphatidylserine + H2O
-
Bacillus cereus phosphorylserine + diacylglycerol
-
?

Synonyms

Synonyms Comment Organism
PC-PLC
-
Bacillus cereus
phospholipase C
-
Bacillus cereus
PLC
-
Bacillus cereus
PLCBC
-
Bacillus cereus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.47
-
3(S),4-dihexanoylbutyl-1-phosphonycholine
-
Bacillus cereus