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Literature summary for 3.1.4.3 extracted from

  • Sonoki, S.; Ikezawa, H.
    Studies on phospholipase C from Pseudomonas aureofaciens. Purification and some properties of phospholipase C (1975), Biochim. Biophys. Acta, 403, 412-424.
    View publication on PubMed
Search for more literature for 3.1.4.3

Activating Compound

Activating Compound Comment Organism Structure
sodium deoxycholate
-
 Pseudomonas chlororaphis subsp. aureofaciens

General Stability

General Stability Organism
1,10-phenanthroline, denaturation Pseudomonas chlororaphis subsp. aureofaciens

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ inhibits phosphatidylethanolamine hydrolysis Pseudomonas chlororaphis subsp. aureofaciens
cetylmethylammonium bromide
-
 Pseudomonas chlororaphis subsp. aureofaciens
Cu2+ 4 mM Pseudomonas chlororaphis subsp. aureofaciens
EDTA
-
 Pseudomonas chlororaphis subsp. aureofaciens
Fe2+ 4 mM Pseudomonas chlororaphis subsp. aureofaciens
Mg2+ inhibits phosphatidylethanolamine hydrolysis Pseudomonas chlororaphis subsp. aureofaciens
Mn2+ inhibits phosphatidylethanolamine hydrolysis Pseudomonas chlororaphis subsp. aureofaciens
o-phenanthroline
-
 Pseudomonas chlororaphis subsp. aureofaciens
Tris buffer in alkaline range Pseudomonas chlororaphis subsp. aureofaciens
Zn2+ 4 mM Pseudomonas chlororaphis subsp. aureofaciens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Pseudomonas chlororaphis subsp. aureofaciens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Pseudomonas chlororaphis subsp. aureofaciens
Mg2+
-
 Pseudomonas chlororaphis subsp. aureofaciens
Mn2+
-
 Pseudomonas chlororaphis subsp. aureofaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 gel filtration Pseudomonas chlororaphis subsp. aureofaciens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphatidylcholine + H2O Pseudomonas chlororaphis subsp. aureofaciens
-
 1,2-diacylglycerol + phosphorylcholine
-
 ?
phosphatidylcholine + H2O Pseudomonas chlororaphis subsp. aureofaciens IFO-3521
-
 1,2-diacylglycerol + phosphorylcholine
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas chlororaphis subsp. aureofaciens
-
-
-
Pseudomonas chlororaphis subsp. aureofaciens IFO-3521
-
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration, ion-exchange chromatography, 3645fold, apparently pure Pseudomonas chlororaphis subsp. aureofaciens

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17.5
-
 substrate: phosphatidylcholine Pseudomonas chlororaphis subsp. aureofaciens

Storage Stability

Storage Stability Organism
-20°C, in Na2B4O7/HCl buffer, pH 7.5, 40% glycerol, no loss of activity after 6 months Pseudomonas chlororaphis subsp. aureofaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lysophosphatidylcholine + H2O
-
 Pseudomonas chlororaphis subsp. aureofaciens monoacylglycerol + phosphorylcholine
-
 ?
lysophosphatidylcholine + H2O
-
 Pseudomonas chlororaphis subsp. aureofaciens IFO-3521 monoacylglycerol + phosphorylcholine
-
 ?
phosphatidylcholine + H2O
-
 Pseudomonas chlororaphis subsp. aureofaciens 1,2-diacylglycerol + phosphorylcholine
-
 ?
phosphatidylcholine + H2O
-
 Pseudomonas chlororaphis subsp. aureofaciens IFO-3521 1,2-diacylglycerol + phosphorylcholine
-
 ?
phosphatidylethanolamine + H2O
-
 Pseudomonas chlororaphis subsp. aureofaciens phosphorylethanolamine + diacylglycerol
-
 ?
phosphatidylethanolamine + H2O
-
 Pseudomonas chlororaphis subsp. aureofaciens IFO-3521 phosphorylethanolamine + diacylglycerol
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 for 10 min, deactivation Pseudomonas chlororaphis subsp. aureofaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 substrate: phosphatidylcholine Pseudomonas chlororaphis subsp. aureofaciens
8 8.5 substrate: phosphatidylethanolamine Pseudomonas chlororaphis subsp. aureofaciens

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 9
-
 Pseudomonas chlororaphis subsp. aureofaciens