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Literature summary for 3.1.4.3 extracted from
- Purification and some properties of phospholipase C (a-toxin) of Clostridium perfringens (1977), J. Biochem., 81, 115-126.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| sodium cholate | - |
Clostridium perfringens |  |
| sodium dehydrocholate | - |
Clostridium perfringens | |
| sodium deoxycholate | at concentrations up to 0.83mg/ml, optimal molar ratio of sodium deoxycholate to lecithin: 0.5 | Clostridium perfringens | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| sodium deoxycholate | at concentrations above 2.5 mg/ml | Clostridium perfringens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Clostridium perfringens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Clostridium perfringens | |
| Co2+ | - |
Clostridium perfringens | |
| Mn2+ | - |
Clostridium perfringens | |
| Zn2+ | - |
Clostridium perfringens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
gel filtration | Clostridium perfringens |
| 43000 | - |
SDS-PAGE | Clostridium perfringens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylcholine + H2O | Clostridium perfringens | - |
1,2-diacylglycerol + phosphorylcholine | - |
? | |
| phosphatidylcholine + H2O | Clostridium perfringens PB6K | - |
1,2-diacylglycerol + phosphorylcholine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium perfringens | - |
- |
- |
| Clostridium perfringens PB6K | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| column chromatography, gel filtration | Clostridium perfringens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1575 | - |
substrate: phosphatidylcholine | Clostridium perfringens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, crude enzyme, stable for more than 6 months | Clostridium perfringens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylcholine + H2O | - |
Clostridium perfringens | 1,2-diacylglycerol + phosphorylcholine | - |
? | |
| phosphatidylcholine + H2O | - |
Clostridium perfringens PB6K | 1,2-diacylglycerol + phosphorylcholine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 31000-43000, gel filtration, SDS-PAGE | Clostridium perfringens |
| polymer | identical subunits of 31000-43000 Da, gel filtration, SDS-PAGE, multiple forms | Clostridium perfringens |
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