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Literature summary for 3.1.4.3 extracted from
- Phospholipase C from Bacillus cereus (1981), Methods Enzymol., 71, 725-730.
No PubMed abstract available
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Bacillus cereus |
General Stability
| General Stability | Organism |
|---|---|
| 1,10-phenanthroline, denaturation | Bacillus cereus |
| urea, PLC is catalytically active in 8 M urea | Bacillus cereus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | in absence of free Zn2+ in solution | Bacillus cereus |  |
| Cu2+ | in absence of free Zn2+ in solution | Bacillus cereus | |
| EDTA | - |
Bacillus cereus | |
| Ni2+ | in absence of free Zn2+ in solution | Bacillus cereus | |
| o-phenanthroline | - |
Bacillus cereus | |
| Urea | denaturation | Bacillus cereus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus cereus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | metalloenzyme | Bacillus cereus | |
| Zn2+ | 2 mol Zn2+ per mol enzyme | Bacillus cereus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
ultracentrifugation, SDS-PAGE, amino acid calculation | Bacillus cereus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylcholine + H2O | Bacillus cereus | - |
1,2-diacylglycerol + phosphorylcholine | - |
? | |
| sphingomyelin + H2O | Bacillus cereus | - |
ceramide + phosphorylcholine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | - |
strain ATCC 10987 AB-1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatography steps, column chromatography | Bacillus cereus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1510 | - |
- |
Bacillus cereus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 1-2 mg purified enzyme/ml, indefinitely stable | Bacillus cereus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylcholine + H2O | - |
Bacillus cereus | 1,2-diacylglycerol + phosphorylcholine | - |
? | |
| phosphatidylethanolamine + H2O | - |
Bacillus cereus | phosphorylethanolamine + diacylglycerol | - |
? | |
| phosphatidylinositol + H2O | - |
Bacillus cereus | inositol monophosphate + diacylglycerol | - |
? | |
| phosphatidylserine + H2O | - |
Bacillus cereus | phosphorylserine + diacylglycerol | - |
? | |
| sphingomyelin + H2O | - |
Bacillus cereus | ceramide + phosphorylcholine | - |
? | |
| sphingomyelin + H2O | when substrate is presented in a biological membrane, for example in the human red cell membrane or myelin sheath | Bacillus cereus | ceramide + phosphorylcholine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 23000, ultracentrifugation, SDS-PAGE, amino acid calculation | Bacillus cereus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | - |
substrate: insoluble long-chain phosphatidylcholine | Bacillus cereus |
| 7.5 | 8 | substrate: water-soluble short-chain phosphatidylcholine | Bacillus cereus |
| 8 | - |
substrate: insoluble long-chain phosphatidylcholine | Bacillus cereus |
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