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Literature summary for 3.1.4.3 extracted from
- Overexpression and biochemical characterization of phospholipase C-beta 1a and -beta 1b (1994), Mol. Cells, 4, 361-367.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| sodium deoxycholate | 1mg/ml | Rattus norvegicus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| transfected into CV-1 cells | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | slightly inhibitory at concentrations of 10 mM | Rattus norvegicus | |
| sodium deoxycholate | at concentrations above 1mg/ml | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.087 | - |
phosphatidylinositol | PLC-beta1a, 2mM free Ca2+ | Rattus norvegicus | |
| 0.137 | - |
phosphatidylinositol 4,5-bisphosphate | PLC-beta1a, 2mM free Ca2+ | Rattus norvegicus | |
| 0.369 | - |
phosphatidylinositol | PLC-beta1b, 2mM free Ca2+ | Rattus norvegicus | |
| 0.571 | - |
phosphatidylinositol 4,5-bisphosphate | PLC-beta1b, 2mM free Ca2+ | Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | PLC-beta 1a and -beta 1b: maximum activity with 3 mM with phosphatidylinositol for substrate, PLC translocation to membranes by free Ca2+ concentration of 100 nM | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 140000 | - |
recombinant PLC -beta 1b, SDS-PAGE | Rattus norvegicus |
| 150000 | - |
recombinant PLC -beta 1a, SDS-PAGE | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylinositol-bisphosphate + H2O | Rattus norvegicus | - |
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant PLC-beta 1a 58fold, recombinant PLC-beta 1b 47fold, HPLC, affinity chromatography, reverse phase chromatography | Rattus norvegicus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.78 | - |
PLC-beta 1b, substrate: phosphatidylinositol | Rattus norvegicus |
| 1.34 | - |
PLC-beta 1a, substrate: phosphatidylinositol | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylinositol + H2O | - |
Rattus norvegicus | inositol monophosphate + diacylglycerol | - |
? | |
| phosphatidylinositol-bisphosphate + H2O | - |
Rattus norvegicus | inositol 1,4,5-trisphosphate + 1,2-diacylglycerol | - |
? | |
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