Cloned (Comment) | Organism |
---|---|
tranformed in Escherichia coli | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Homo sapiens | |
Ca2+ | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85100 | - |
PLC delta 3, gel electrophoresis | Homo sapiens |
85500 | - |
PLC delta 1, gel electrophoresis | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylinositol-bisphosphate + H2O | Homo sapiens | - |
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol | - |
? | |
phosphatidylinositol-bisphosphate + H2O | Rattus norvegicus | - |
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant PLC d1 and D3 with poly-His-tail at the amino terminus, metal ion affinity chromatography | Homo sapiens |
recombinant PLC d1 and D3 without poly-His-tail at the amino terminus, cation exchange HPLC and phosphocellulose chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
404 | - |
substrate: phosphatidylinositol-4,5-bisphosphate, PLC d3 with poly-His-tail, no significant change of activity after cleavage of poly-His-tail, expression at 16°C for 72 h | Homo sapiens |
1268 | - |
substrate: phosphatidylinositol-4,5-bisphosphate, PLC d1 with poly-His-tail, no significant change of activity after cleavage of poly-His-tail, expression at 16°C for 72 h | Homo sapiens |
Storage Stability | Organism |
---|---|
-20°C, buffer B, purified recombinant PLC d1 and d3 with poly-His-tail, activity stable over 2 years, more than 15 freeze/thaw cycles between -20°C and 4°C reduce activity by 10-20% | Homo sapiens |
-20°C, crude extract, enzyme activity stable over 2 years | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylinositol-bisphosphate + H2O | - |
Homo sapiens | inositol 1,4,5-trisphosphate + 1,2-diacylglycerol | - |
? | |
phosphatidylinositol-bisphosphate + H2O | - |
Rattus norvegicus | inositol 1,4,5-trisphosphate + 1,2-diacylglycerol | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | - |
for 60 min, recombinant PLC delta 1 and delta 3 with poly-His-tail, no loss of activity | Homo sapiens |
37 | - |
for 30 min, recombinant PLC delta 1 and delta 3 with poly-His-tail, no loss of activity | Homo sapiens |