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Literature summary for 3.1.4.3 extracted from
- Phospholipases (1971), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 5, 71-85.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| diethyl ether | substrate: sphingomyelin | Clostridium perfringens |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | substrate: sphingomyelin | Clostridium perfringens | |
| Ca2+ | substrate: sphingomyelin | Clostridium perfringens | |
| Cu2+ | - |
Bacillus cereus | |
| Cu2+ | substrate: sphingomyelin | Clostridium perfringens | |
| dithiothreitol | - |
Bacillus cereus | |
| EDTA | - |
Bacillus cereus | |
| EDTA | substrate: sphingomyelin | Clostridium perfringens | |
| Fe2+ | substrate: sphingomyelin | Clostridium perfringens | |
| Zn2+ | substrate: sphingomyelin | Clostridium perfringens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
sphingomyelin | - |
Clostridium perfringens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus cereus | - |
- |
| extracellular | - |
Clostridium perfringens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Clostridium perfringens | |
| Mg2+ | restores ability of Ca2+-inhibited PLC to hydrolyse sphingomyelin | Clostridium perfringens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylcholine + H2O | Bacillus cereus | - |
1,2-diacylglycerol + phosphorylcholine | - |
? | |
| phosphatidylcholine + H2O | Clostridium perfringens | - |
1,2-diacylglycerol + phosphorylcholine | - |
? | |
| phosphatidylcholine + H2O | Clostridium welchii | - |
1,2-diacylglycerol + phosphorylcholine | - |
? | |
| phosphatidylcholine + H2O | Bacillus cereus 7004 | - |
1,2-diacylglycerol + phosphorylcholine | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| diethyl ether | activating | Clostridium perfringens |
| mercaptoethanol | inhibitory | Clostridium perfringens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | - |
- |
- |
| Bacillus cereus 7004 | - |
- |
- |
| Clostridium perfringens | - |
strain ATCC 10543 | - |
| Clostridium welchii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, column chromatography steps | Clostridium perfringens |
| dialysis, column chromatography, 22fold | Bacillus cereus |
| protamine precipitation, column chromatography, 23fold | Bacillus cereus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diacylglycerylphosphoryl monomethylethanolamine + H2O | - |
Bacillus cereus | ? | - |
? | |
| diacylglycerylphosphoryl monomethylethanolamine + H2O | - |
Bacillus cereus 7004 | ? | - |
? | |
| lysophosphatidylcholine + H2O | - |
Clostridium perfringens | monoacylglycerol + phosphorylcholine | - |
? | |
| phosphatidylcholine + H2O | - |
Bacillus cereus | 1,2-diacylglycerol + phosphorylcholine | - |
? | |
| phosphatidylcholine + H2O | - |
Clostridium perfringens | 1,2-diacylglycerol + phosphorylcholine | - |
? | |
| phosphatidylcholine + H2O | - |
Clostridium welchii | 1,2-diacylglycerol + phosphorylcholine | - |
? | |
| phosphatidylcholine + H2O | - |
Bacillus cereus 7004 | 1,2-diacylglycerol + phosphorylcholine | - |
? | |
| phosphatidylethanolamine + H2O | - |
Bacillus cereus | phosphorylethanolamine + diacylglycerol | - |
? | |
| phosphatidylethanolamine + H2O | - |
Clostridium perfringens | phosphorylethanolamine + diacylglycerol | - |
? | |
| phosphatidylethanolamine + H2O | - |
Bacillus cereus 7004 | phosphorylethanolamine + diacylglycerol | - |
? | |
| sphingomyelin + H2O | - |
Bacillus cereus | ceramide + phosphorylcholine | - |
? | |
| sphingomyelin + H2O | - |
Clostridium perfringens | ceramide + phosphorylcholine | - |
? | |
| sphingomyelin + H2O | - |
Bacillus cereus 7004 | ceramide + phosphorylcholine | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 8.8 | substrate: sphingomyelin | Clostridium perfringens |
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