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Literature summary for 3.1.4.17 extracted from

  • Lim, S.T.; Krishnan, A.K.; Ong, K.K.
    Purification and characterisation of the second form of cyclic 3',5'-nucleotide phosphodiesterase from Rhizobium fredii (1989), Biochim. Biophys. Acta, 991, 353-358.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol stimulates Sinorhizobium fredii
ascorbic acid stimulates Sinorhizobium fredii
dithiothreitol stimulates Sinorhizobium fredii
glutathione reduced, stimulates Sinorhizobium fredii

Inhibitors

Inhibitors Comment Organism Structure
Al3+ strongly inhibitory at 2.5 mM Sinorhizobium fredii
Fe2+ strongly inhibitory at 2.5 mM Sinorhizobium fredii
Fe3+ strongly inhibitory at 2.5 mM Sinorhizobium fredii
Zn2+ strongly inhibitory at 2.5 mM Sinorhizobium fredii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0018
-
3',5'-cAMP
-
Sinorhizobium fredii
0.002
-
3',5'-cGMP
-
Sinorhizobium fredii

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 40% stimulation at 0.25 mM Sinorhizobium fredii
Ca2+ 40% stimulation at 0.25 mM Sinorhizobium fredii
Co2+ stimulates at 2.5 mM Sinorhizobium fredii
Mg2+ stimulates at 2.5 mM Sinorhizobium fredii
Mn2+ stimulates at 2.5 mM Sinorhizobium fredii
additional information no metal ion required Sinorhizobium fredii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
x * 28000 Sinorhizobium fredii
82000
-
Rhizobium fredii MAR-1, gel filtration Sinorhizobium fredii

Organism

Organism UniProt Comment Textmining
Sinorhizobium fredii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
95.7fold Sinorhizobium fredii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.096
-
-
Sinorhizobium fredii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3',5'-cAMP + H2O
-
Sinorhizobium fredii 5'-AMP
-
?
3',5'-cGMP + H2O
-
Sinorhizobium fredii 5'-GMP
-
?
nucleoside 3',5'-cyclic phosphate + H2O
-
Sinorhizobium fredii nucleoside 5'-phosphate
-
?

Subunits

Subunits Comment Organism
oligomer SDS-PAGE Sinorhizobium fredii
oligomer x * 28000 Sinorhizobium fredii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
28
-
80% of activity remaining after 7 days Sinorhizobium fredii
60
-
20 min, no loss of activity Sinorhizobium fredii
70
-
20 min, 20% loss of activity Sinorhizobium fredii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
Sinorhizobium fredii