Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | stimulates | Sinorhizobium fredii | |
ascorbic acid | stimulates | Sinorhizobium fredii | |
dithiothreitol | stimulates | Sinorhizobium fredii | |
glutathione | reduced, stimulates | Sinorhizobium fredii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | strongly inhibitory at 2.5 mM | Sinorhizobium fredii | |
Fe2+ | strongly inhibitory at 2.5 mM | Sinorhizobium fredii | |
Fe3+ | strongly inhibitory at 2.5 mM | Sinorhizobium fredii | |
Zn2+ | strongly inhibitory at 2.5 mM | Sinorhizobium fredii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0018 | - |
3',5'-cAMP | - |
Sinorhizobium fredii | |
0.002 | - |
3',5'-cGMP | - |
Sinorhizobium fredii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 40% stimulation at 0.25 mM | Sinorhizobium fredii | |
Ca2+ | 40% stimulation at 0.25 mM | Sinorhizobium fredii | |
Co2+ | stimulates at 2.5 mM | Sinorhizobium fredii | |
Mg2+ | stimulates at 2.5 mM | Sinorhizobium fredii | |
Mn2+ | stimulates at 2.5 mM | Sinorhizobium fredii | |
additional information | no metal ion required | Sinorhizobium fredii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
x * 28000 | Sinorhizobium fredii |
82000 | - |
Rhizobium fredii MAR-1, gel filtration | Sinorhizobium fredii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sinorhizobium fredii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
95.7fold | Sinorhizobium fredii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.096 | - |
- |
Sinorhizobium fredii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3',5'-cAMP + H2O | - |
Sinorhizobium fredii | 5'-AMP | - |
? | |
3',5'-cGMP + H2O | - |
Sinorhizobium fredii | 5'-GMP | - |
? | |
nucleoside 3',5'-cyclic phosphate + H2O | - |
Sinorhizobium fredii | nucleoside 5'-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | SDS-PAGE | Sinorhizobium fredii |
oligomer | x * 28000 | Sinorhizobium fredii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
80% of activity remaining after 7 days | Sinorhizobium fredii |
60 | - |
20 min, no loss of activity | Sinorhizobium fredii |
70 | - |
20 min, 20% loss of activity | Sinorhizobium fredii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Sinorhizobium fredii |