Any feedback?
Literature summary for 3.1.4.12 extracted from
- Enzymic hydrolysis of sphingomyelin by rat liver (1966), Biochem. J., 98, 763-769.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cetyltrimethylammonium bromide | low concentrations | Rattus norvegicus |  |
| Detergents | - |
Rattus norvegicus | |
| Triton X-100 | - |
Rattus norvegicus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ceramide | 23% inhibition | Rattus norvegicus | |
| cetyltrimethylammonium bromide | - |
Rattus norvegicus | |
| phosphorylcholine | 23% inhibition | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | 0.9 | sphingomyelin | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| lysosome | - |
Rattus norvegicus | 5764 | - |
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sphingomyelin + H2O | Rattus norvegicus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, 21fold | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -18°C, 0.25 M sucrose, or 20% glycerol, or 33% (NH4)2SO4 | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sphingomyelin + H2O | erythro-isomers of DL-trans-2-N-palmitoyl-1-O-phosphorylcholinesphingosine or dihydrosphingosine hydrolyzed faster than the threo-isomer | Rattus norvegicus | N-acylsphingosine + choline phosphate | - |
? | |
| sphingomyelin + H2O | - |
Rattus norvegicus | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
inactive after 10 min | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 5.5 | depending on buffer | Rattus norvegicus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
