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Literature summary for 3.1.4.11 extracted from

  • De Los Santos-Briones, C.; Cardenas, L.; Estrada-Navarrete, G.; Santana, O.; Minero-Garcia, Y.; Quinto, C.; Sanchez, F.; Nissen, P.
    GTPgammaS antagonizes the mastoparan-induced in vitro activity of PIP-phospholipase C from symbiotic root nodules of Phaseolus vulgaris (2009), Physiol. Plant., 135, 237-245.
    View publication on PubMed
Search for more literature for 3.1.4.11

Activating Compound

Activating Compound Comment Organism Structure
mastoparan activity almost doubled in the presence of 5 microM Phaseolus vulgaris
additional information assay of the effect of Nod factors on in vitro PLC activity from nodule membrane fractions in the presence of guanosine 5'-[gamma-thio]triphosphate (GTPgammaS) and mastoparan Phaseolus vulgaris

Inhibitors

Inhibitors Comment Organism Structure
guanosine 5'-[gamma-thio]triphosphate activity is downregulated by 1-10 mM guanosine 5'-[gamma-thio]triphosphate Phaseolus vulgaris
nod factor activity is decreased by up to 64% in the presence of increasing concentrations of Nod factors Phaseolus vulgaris
U-73122
-
 Phaseolus vulgaris

Organism

Organism UniProt Comment Textmining
Phaseolus vulgaris
-
-
-

Reaction

Reaction Comment Organism Reaction ID
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol biochemical relationship between PLC and heterotrimeric G proteins during NFs signaling in legumes Phaseolus vulgaris
a

Source Tissue

Source Tissue Comment Organism Textmining
root hair
-
 Phaseolus vulgaris
-
root nodule
-
 Phaseolus vulgaris
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.027
-
 specific activity of 20-27 nmol/mg/min in membrane fractions of nodules 18-20 days after inoculation with Rhizobium tropici. In the presence of 5 mM mastoparan, PIP2-PLC activity is almost double the basal level Phaseolus vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
-
 Phaseolus vulgaris 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
 ?

Synonyms

Synonyms Comment Organism
phosphatidylinositol 4,5 bisphosphate-phospholipase C
-
 Phaseolus vulgaris
PIP2-phospholipase C
-
 Phaseolus vulgaris
PIP2-PLC
-
 Phaseolus vulgaris