Any feedback?
Literature summary for 3.1.4.11 extracted from
- A Toxoplasma gondii phosphoinositide phospholipase C (TgPI-PLC) with high affinity for phosphatidylinositol (2006), Biochem. J., 394, 417-425.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21 | Toxoplasma gondii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | when phosphatidylinositol is used as a substrate, the enzyme activity increases with increasing free Ca2+ concentrations above 1 µM, and the activity decreases when free Ca2+-concentration exceeded 1 mM. When 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate is used as substrate, the enzyme is activated at lower Ca2+-concentrations and its activity increases with increasing free Ca2+ concentration. Addition of 3 mM MgCl2 stimulates hydrolysis 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate and inhibites hydrolysis of phosphatidylinositol | Toxoplasma gondii |  |
| Mg2+ | hydrolysis of phosphatidylinositol | Toxoplasma gondii | |
| neomycin | hydrolysis of phosphatidylinositol and 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | Toxoplasma gondii | |
| substance 48/80 | inhibition is more pronounced with phosphatidylinositol as substrate than with 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | Toxoplasma gondii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.079 | - |
phosphatidylinositol | recombinant enzyme | Toxoplasma gondii | |
| 0.13 | - |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | recombinant enzyme | Toxoplasma gondii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plasma membrane | extracellular phase of plasma membrane | Toxoplasma gondii | 5886 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | when phosphatidylinositol is used as a substrate, the enzyme activity increases with increasing free Ca2+ concentrations above 0.001 mM, and the activity decreases when free Ca2+-concentration exceeded 1 mM. When 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate is used as substrate, the enzyme is activated at lower Ca2+-concentrations and its activity increases with increasing free Ca2+ concentration. Addition of 3 mM MgCl2 stimulates hydrolysis of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate and inhibites hydrolysis of phosphatidylinositol | Toxoplasma gondii | |
| Mg2+ | slight stimulation of hydrolysis of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | Toxoplasma gondii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 121000 | - |
calculated from sequence | Toxoplasma gondii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Toxoplasma gondii | Q3ZTU7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Toxoplasma gondii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| tachyzoite | - |
Toxoplasma gondii | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O | - |
Toxoplasma gondii | 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol | - |
? | |
| phosphatidylinositol + H2O | - |
Toxoplasma gondii | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TgPI-PLC | - |
Toxoplasma gondii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
hydrolysis of phosphatidylinositol | Toxoplasma gondii |
| 30 | - |
hydrolysis of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | Toxoplasma gondii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 30 | 5°C: about 50% of maximal activity, 30°C: about 45% of maximal activity, hydrolysis of phosphatidylinositol | Toxoplasma gondii |
| 15 | 37 | 15°C: about 75% of maximal activity, 37°C: about 80% of maximal activity, hydrolysis of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | Toxoplasma gondii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 7.5 | hydrolysis of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | Toxoplasma gondii |
| 8 | - |
hydrolysis of phosphatidylinositol | Toxoplasma gondii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.1 | 7.6 | pH 7.1: about 85% of maximal activity, pH 7.6: about 65% of maximal activity, hydrolysis of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | Toxoplasma gondii |
| 7.1 | 9.2 | pH 7.1: about 90% of maximal activity, pH 9.2: about 85% of maximal activity, hydrolysis of phosphatidylinositol | Toxoplasma gondii |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
