Cloned (Comment) | Organism |
---|---|
wild-type and mutant PLCgamma2, expression in DT40 cells, A431 cells and in Sf9 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
T753F | PLCgamma2 mutant | Homo sapiens |
T753F/T759F | active PLCgamma2 double mutant with a loss of tyrosine phosphorylation | Homo sapiens |
T759F | PLCgamma2 mutant | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | PLCgamma2, Ca2+-dependent, highest activity at 0.005-0.01 mM | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | PLCgamma isoforms are regulated through activation of tyrosine kinase-linked receptors, importance of PLCgamma2 in B-cell signal transduction | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P16885 | PLCgamma2 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | stimulation of PLCgamma2 activity by tyrosine phosphorylation, multiple phosphorylation sites, the kinase Btk phosphorylates recombinant PLCgamma2 in vitro, Tyr-753 and Tyr-759 are essential for PLCgamma2 function in B-cells, preferential phosphorylation of Tyr-753 over Tyr-759 | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant PLCgamma2, expressed in Sf9 cells | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O | - |
Homo sapiens | 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol | - |
? | |
additional information | PLCgamma isoforms are regulated through activation of tyrosine kinase-linked receptors, importance of PLCgamma2 in B-cell signal transduction | Homo sapiens | ? | - |
? |