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Literature summary for 3.1.4.11 extracted from

  • Furuya, T.; Kashuba, C.; Docampo, R.; Moreno, S.N.J.
    A novel phosphatidylinositol-phospholipase C of Trypanosoma cruzi that is lipid modified and activated during trypomastigote to amastigote differentiation (2000), J. Biol. Chem., 275, 6428-6438.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information TcPI-PLC expression is induced during the differentiation of trypomastigotes into amastigotes Trypanosoma cruzi

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3), sequencing Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate pH 7.1, 30°C Trypanosoma cruzi

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane associated with, in amastigotes Trypanosoma cruzi 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ dependent on free Ca2+, enzyme needs a higher calcium concentration for its activation with phosphatidylinositol as substrate than with phosphatidylinositol 4,5-bisphosphate Trypanosoma cruzi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
x * 82000, calculated from the amino acid sequence, x * 80000, SDS-PAGE, Western blot analysis Trypanosoma cruzi
82000
-
x * 82000, calculated from the amino acid sequence, x * 80000, SDS-PAGE, Western blot analysis Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O Trypanosoma cruzi inositol phosphate/diacylglycerol signaling pathway, TcPI-PLC is lipid modified in vivo, TcPI-PLC is developmentally regulated, its expression is induced during the differentiation of trypomastigotes into amastigotes at an early stage in the transformation process 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
?

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi Q9TZN8
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
lipoprotein TcPI-PLC is lipid modified in vivo, TcPI-PLC contains an N-myristoylation consensus sequence at its N-terminal end and is modified by myristate and palmitate, myristoyl group may play an important role in the association of enzyme with the plasma membrane Trypanosoma cruzi

Purification (Commentary)

Purification (Comment) Organism
recombinant TcPI-PLC Trypanosoma cruzi

Source Tissue

Source Tissue Comment Organism Textmining
amastigote TcPI-PLC expression at high levels in the epimastigote and amastigote stages Trypanosoma cruzi
-
epimastigote TcPI-PLC expression at high levels in the epimastigote and amastigote stages Trypanosoma cruzi
-
trypomastigote lower TcPI-PLC expression than in epimastigotes and amastigotes Trypanosoma cruzi
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O phosphoinositide-specific, preferred substrate to phosphatidylinositol, domain structure Trypanosoma cruzi 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O inositol phosphate/diacylglycerol signaling pathway, TcPI-PLC is lipid modified in vivo, TcPI-PLC is developmentally regulated, its expression is induced during the differentiation of trypomastigotes into amastigotes at an early stage in the transformation process Trypanosoma cruzi 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
?
phosphatidylinositol + H2O phosphatidylinositol 4,5-bisphosphate is preferred to phosphatidylinositol Trypanosoma cruzi myo-inositol 1-phosphate + diacylglycerol
-
?

Subunits

Subunits Comment Organism
? x * 82000, calculated from the amino acid sequence, x * 80000, SDS-PAGE, Western blot analysis Trypanosoma cruzi

Synonyms

Synonyms Comment Organism
TcPI-PLC PI-PLC of Trypanosoma cruzi, similar to mamalian delta-type PI-PLCs Trypanosoma cruzi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Trypanosoma cruzi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7 6.8
-
Trypanosoma cruzi

pH Range

pH Minimum pH Maximum Comment Organism
6.5 7.3 enzyme activity decreases very rapidly below pH 6.5 and above pH 7.25 Trypanosoma cruzi