Cloned (Comment) | Organism |
---|---|
gene At5g15170 or AtTDP, DNA and amino acid sequence determination and analysis, expression of GFP-tagged AtTDP in Arabidopsis thaliana plants | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | loss-of-function AtTDP mutation , genomic structure of the tdp T-DNA insertion mutant, overview. The tdp Dwarf mutant is caused by reduced cell numbers, phenotype, overview | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Arabidopsis thaliana | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arabidopsis thaliana | AtTDP has Tyr-phosphodiesterase activity for the topoisomerase-DNA complex | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q8H1D9 | gene At5g15170 or AtTDP | - |
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | AtTDP has Tyr-phosphodiesterase activity for the topoisomerase-DNA complex | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TDP1 | - |
Homo sapiens |
TDP1 | - |
Arabidopsis thaliana |
tyrosyl-DNA phosphodiesterase 1 | - |
Homo sapiens |
tyrosyl-DNA phosphodiesterase 1 | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | a loss-of-function AtTDP mutation displays developmental defects and dwarf phenotype in Arabidopsis. This phenotype is substantially caused by decreased cell numbers without any change of individual cell sizes. The tdp plants exhibit hypersensitivities to camptothecin, a potent topoisomerase I inhibitor, and show rigorous cell death in cotyledons and rosette leaves, suggesting the failure of DNA damage repair in tdp mutants | Arabidopsis thaliana |
malfunction | mutations of the Tdp1 gene are involved in the disease spinocerebellar ataxia with axonal neuropathy | Homo sapiens |
physiological function | AtTDP plays a clear role in the repair of topoisomerase I-DNA complexes in Arabidopsis, the tyrosyl-DNA phosphodiesterase 1 is a key enzyme that hydrolyzes the phosphodiester bond between tyrosine of topoisomerase and 3'-phosphate of DNA and repairs topoisomerase-mediated DNA damage during chromosome metabolism. Recombinant AtTDP protein certainly hydrolyzes the 3'-phosphotyrosyl DNA substrates related to repairing in vivo topoisomerase I-DNA-induced damage | Arabidopsis thaliana |
physiological function | tyrosyl-DNA phosphodiesterase 1 is a key enzyme that hydrolyzes the phosphodiester bond between tyrosine of topoisomerase and 3'-phosphate of DNA and repairs topoisomerase-mediated DNA damage during chromosome metabolism | Homo sapiens |