Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.4.1 extracted from

  • Banerjee, B.; Roy, A.; Sen, N.; Majumder, H.K.
    A tyrosyl DNA phosphodiesterase 1 from kinetoplastid parasite Leishmania donovani (LdTdp1) capable of removing topo I-DNA covalent complexes (2010), Mol. Microbiol., 78, 119-137.
    View publication on PubMed
Search for more literature for 3.1.4.1

Cloned(Commentary)

Cloned (Comment) Organism
gene LdTdp1, DNA and amino acid sequence determination and analysis Leishmania donovani

Protein Variants

Protein Variants Comment Organism
additional information construction of a series of N-terminal deletion constructs of LdTdp1 with a GFP fused at the C-terminus, expression in Leishmania donovani. This C-terminal deletion construct exhibits cytoplasmic distribution of green fluorescence Leishmania donovani

Localization

Localization Comment Organism GeneOntology No. Textmining
kinetoplast
-
 Leishmania donovani 20023
-
nucleus the enzyme harbours a nuclear localization signal at its C-terminus Leishmania donovani 5634
-

Organism

Organism UniProt Comment Textmining
Leishmania donovani C8CT07
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information product of the reaction is a 3'-phosphate and not a 3'-hydroxyl Leishmania donovani ?
-
 ?

Synonyms

Synonyms Comment Organism
More tyrosyl DNA phosphodiesterase 1 is a member of phospholipase D, PLD, superfamily Leishmania donovani
TDP1
-
 Leishmania donovani
tyrosyl DNA phosphodiesterase 1
-
 Leishmania donovani

General Information

General Information Comment Organism
evolution conservation of the active site motifs typical for all Tdp1 proteins Leishmania donovani
additional information overexpression of the active enzyme protects the parasites against topoisomerase IB inhibitor camptothecin and oxidative agent H2O2-mediated cytotoxicity and its downregulation rendered the parasites hypersensitive to camptothecin. Downregulation of LdTdp1 mRNA occurs because of blockage of transcription rather than increased turnover, transcriptional regulation, overview Leishmania donovani
physiological function th enzyme participates in topoisomerase I-mediated DNA damage repair process and thereby counteracts the cytotoxic effect of topoisomerase I inhibitors Leishmania donovani