Crystallization (Comment) | Organism |
---|---|
2.25 A resolution, in presence and absence of sulfate inhibitor. Protein has a beta-sandwich architecture and is a tetramer with two zinc ions per active site | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.74 | - |
bis-p-nitrophenyl phosphate | pH 8.5, 37°C | Escherichia coli | |
18.3 | - |
bis-p-nitrophenyl phosphate | pH 8.5, 37°C, presence of 0.5 mM phosphate | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | KM value 0.022 mM | Escherichia coli | |
Zn2+ | enzyme carries two zinc ions per active site | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
79400 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bis-p-nitrophenyl phosphate + H2O | - |
Escherichia coli | p-nitrophenyl phosphate + nitrophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | crystallitazion data | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
yfcE | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
19.8 | - |
bis-p-nitrophenyl phosphate | pH 8.5, 37°C | Escherichia coli | |
20.3 | - |
bis-p-nitrophenyl phosphate | pH 8.5, 37°C, presence of 0.5 mM phosphate | Escherichia coli |