Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.4.1 extracted from

  • Keppetipola, N.; Shuman, S.
    Characterization of the 2,3 cyclic phosphodiesterase activities of Clostridium thermocellum polynucleotide kinase-phosphatase and bacteriophage lambda phosphatase (2007), Nucleic Acids Res., 35, 7721-7732.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.1

Protein Variants

Protein Variants Comment Organism
D236A strong decrease in KM value Acetivibrio thermocellus
D236N strong decrease in KM value Acetivibrio thermocellus
D392E transformation of metal specificity, mutant is a Mn2+-dependent phosphodiesterase/monoesterase Acetivibrio thermocellus
D392N transformation of metal and substrate specificity, mutant is a Mn2+-dependent phosphodiesterase Acetivibrio thermocellus
H189A transformation of metal and substrate specificity, mutant is a Mn2+-dependent phosphodiesterase Acetivibrio thermocellus
H189D transformation of metal and substrate specificity, mutant is a Mn2+-dependent phosphodiesterase. Strong stimulation of activity Acetivibrio thermocellus
H189E transformation of substrate specificity, mutant is a Mn2+/Ni2+-dependent phosphodiesterase Acetivibrio thermocellus
H189Q decrease in phosphodiesterase activity Acetivibrio thermocellus
H264A strong stimulation of phosphodiesterase activity Acetivibrio thermocellus
H264N strong stimulation of phosphodiesterase activity Acetivibrio thermocellus
H264Q strong stimulation of phosphodiesterase activity Acetivibrio thermocellus
H376D transformation of metal specificity, mutant is a Mn2+-dependent phosphodiesterase/monoesterase Acetivibrio thermocellus
H376N transformation of metal specificity, mutant is a Mn2+-dependent phosphodiesterase/monoesterase Acetivibrio thermocellus
R237A strong stimulation of phosphodiesterase activity Acetivibrio thermocellus
R237E strong stimulation of phosphodiesterase activity Acetivibrio thermocellus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ Mn2+-dependent phophomonoesterase activity requires two additional amino acid residues compared with Ni2+-dependent phosphomonoester activity. Mn2+-dependent phosphodiesterase activity requires one amino acid residues less than Ni2+-dependent phosphodiesterase activity Acetivibrio thermocellus
Ni2+ both Ni2+-dependent phophomonoesterase activity and Ni2+-dependent phosphodiesterase activity require the same seven amino acid residues of the enzyme Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
 bifunctional phosphodiesterase/phosphomonoesterase
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',3'-cAMP + H2O
-
 Acetivibrio thermocellus ?
-
 ?
bis-p-nitrophenyl phosphate + H2O
-
 Acetivibrio thermocellus p-nitrophenol + p-nitrophenyl phosphate processive two-step mechanism ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.9
-
 2',3'-cAMP pH 8.0, 45°C, mutant H264Q, presence of Ni2+ Acetivibrio thermocellus
1.7
-
 2',3'-cAMP pH 8.0, 45°C, wild-type, presence of Ni2+ Acetivibrio thermocellus
2.1
-
 2',3'-cAMP pH 8.0, 45°C, mutant H376D, presence of Mn2+ Acetivibrio thermocellus
2.5
-
 2',3'-cAMP pH 8.0, 45°C, mutant D236A, presence of Ni2+ Acetivibrio thermocellus
3 6 2',3'-cAMP pH 8.0, 45°C, mutant H189D, presence of Mn2+ Acetivibrio thermocellus
3.3
-
 2',3'-cAMP pH 8.0, 45°C, mutant H189Q, presence of Mn2+ Acetivibrio thermocellus
3.6
-
 2',3'-cAMP pH 8.0, 45°C, mutant R237K, presence of Ni2+ Acetivibrio thermocellus
3.7
-
 2',3'-cAMP pH 8.0, 45°C, mutant H189A, presence of Mn2+ Acetivibrio thermocellus
8.8
-
 2',3'-cAMP pH 8.0, 45°C, mutant D392E, presence of Mn2+ Acetivibrio thermocellus
9.3
-
 2',3'-cAMP pH 8.0, 45°C, mutant D236N, presence of Ni2+ Acetivibrio thermocellus
12
-
 2',3'-cAMP pH 8.0, 45°C, mutant H264N, presence of Ni2+ Acetivibrio thermocellus
13.8
-
 2',3'-cAMP pH 8.0, 45°C, mutant D392N, presence of Mn2+ Acetivibrio thermocellus
18
-
 2',3'-cAMP pH 8.0, 45°C, mutant H264A, presence of Ni2+ Acetivibrio thermocellus
19.3
-
 2',3'-cAMP pH 8.0, 45°C, mutant R237A, presence of Ni2+ Acetivibrio thermocellus
23.2
-
 2',3'-cAMP pH 8.0, 45°C, wild-type, presence of Mn2+ Acetivibrio thermocellus
33
-
 2',3'-cAMP pH 8.0, 45°C, mutant R237Q, presence of Ni2+ Acetivibrio thermocellus
40.5
-
 2',3'-cAMP pH 8.0, 45°C, mutant H376N, presence of Mn2+ Acetivibrio thermocellus
43.5
-
 2',3'-cAMP pH 8.0, 45°C, mutant D236A, presence of Mn2+ Acetivibrio thermocellus
52.3
-
 2',3'-cAMP pH 8.0, 45°C, mutant H264D, presence of Mn2+ Acetivibrio thermocellus
65.9
-
 2',3'-cAMP pH 8.0, 45°C, mutant D236N, presence of Mn2+ Acetivibrio thermocellus
117.5
-
 2',3'-cAMP pH 8.0, 45°C, mutant H189E, presence of Mn2+ Acetivibrio thermocellus
253
-
 2',3'-cAMP pH 8.0, 45°C, mutant H264Q, presence of Mn2+ Acetivibrio thermocellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 phosphomonoesterase activity, assay at Acetivibrio thermocellus
8
-
 phosphodiesterase activity, assay at Acetivibrio thermocellus