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Literature summary for 3.1.4.1 extracted from

  • Garcia-Nieto, R.M.; Jose, E.S.; Martin-Nieto, J.; Villalobo, A.
    Characterization of a new plasma membrane-associated ecto-5'-phosphodiesterase/nucleotide-pyrophosphatase from rat hepatocarcinoma AS-30D cells (2001), J. Physiol. Biochem., 57, 31-40.
    View publication on PubMed
Search for more literature for 3.1.4.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.097
-
 thymidine 5'-monophosphate p-nitrophenylester pH 9.6, gp115, AS30D protein Rattus norvegicus
0.22
-
 thymidine 5'-monophosphate p-nitrophenylester pH 9.6, gp120/gp110, liver protein Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required, both isozymes Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
 x * 115000, gp115, and x * 110000 + x * 120000, gp120/gp110 Rattus norvegicus
115000
-
 x * 115000, gp115, and x * 110000 + x * 120000, gp120/gp110 Rattus norvegicus
120000
-
 x * 115000, gp115, and x * 110000 + x * 120000, gp120/gp110 Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 two isozymes
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein both isozymes Rattus norvegicus
phosphoprotein gp115 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
AS-30D cell hepatocarcinoma cell line Rattus norvegicus
-
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
thymidine 5'-monophosphate p-nitrophenylester + H2O
-
 Rattus norvegicus p-nitrophenol + TMP
-
 ?

Subunits

Subunits Comment Organism
? x * 115000, gp115, and x * 110000 + x * 120000, gp120/gp110 Rattus norvegicus