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Literature summary for 3.1.4.1 extracted from

  • Kelly, S.J.; Butler, L.G.
    Enzymic hydrolysis of phosphonate esters. Reaction mechanism of intestinal 5'-nucleotide phosphodiesterase (1977), Biochemistry, 16, 1102-1104.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
AMP 5'-AMP, competitive Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
NAD+
-
Bos taurus
0.043
-
p-nitrophenylthymidine 5'-phosphate
-
Bos taurus
0.1
-
3',5'-cAMP
-
Bos taurus
0.2
-
ATP
-
Bos taurus
0.85
-
bis(p-nitrophenyl) phosphate
-
Bos taurus
11
-
p-Nitrophenyl phenylphosphonate
-
Bos taurus
18
-
2-naphthyl phenylphosphonate
-
Bos taurus
41
-
p-nitrophenyl chloromethylphosphonate
-
Bos taurus
66
-
p-nitrophenyl methylphosphonate
-
Bos taurus
83
-
2-naphthyl chloromethylphosphonate
-
Bos taurus
120
-
2-naphthyl methylphosphonate
-
Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
[nucleotide]n + H2O = [nucleotide]m + nucleotide mechanism Bos taurus
[nucleotide]n + H2O = [nucleotide]m + nucleotide ping-pong type mechanism, with participation of a covalent enzyme intermediate Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
intestine
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
47.7
-
-
Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-naphthyl chloromethylphosphonate + H2O
-
Bos taurus 2-naphthol + chloromethylphosphonic acid
-
?
2-naphthyl methylphosphonate + H2O
-
Bos taurus 2-naphthol + methylphosphonic acid
-
?
2-naphthyl phenylphosphonate + H2O
-
Bos taurus 2-naphthol + benzenephosphonic acid
-
?
ADP + H2O slowly Bos taurus AMP + phosphate
-
?
ATP + H2O slowly Bos taurus 5'-AMP + diphosphate
-
?
bis(p-nitrophenyl) phosphate + H2O
-
Bos taurus ?
-
?
cAMP + H2O
-
Bos taurus ?
-
?
cyclic 3',5'-mononucleotides + H2O 3',5'-cAMP, slowly Bos taurus mononucleoside 5'-phosphate
-
?
NAD+ + H2O slowly Bos taurus 5'-AMP + NMN
-
?
p-nitrophenyl chloromethylphosphonate + H2O
-
Bos taurus p-nitrophenol + chloromethylphosphonic acid
-
?
p-nitrophenyl methylphosphonate + H2O
-
Bos taurus p-nitrophenol + methylphosphonic acid
-
?
p-nitrophenyl phenylphosphonate + H2O
-
Bos taurus p-nitrophenol + benzenephosphonic acid
-
?
p-nitrophenylthymidine 5'-phosphate + H2O
-
Bos taurus 5'-TMP + p-nitrophenol
-
?
phosphonate ester + H2O
-
Bos taurus ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * ?, identical dimer Bos taurus