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Literature summary for 3.1.4.1 extracted from

  • Luthje, J.; Ogilvie, A.
    Catabolism of Ap3A and Ap4A in human plasma. Purification and characterization of a glycoprotein complex with 5'-nucleotide phosphodiesterase activity (1985), Eur. J. Biochem., 149, 119-127.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
5'-nucleotides
-
Homo sapiens
ADP
-
Homo sapiens
AMP
-
Homo sapiens
CMP
-
Homo sapiens
CoA
-
Homo sapiens
EDTA completely, inactivation reversed by Ca2+, Co2+ and, at best, with Zn2+ Homo sapiens
GTP
-
Homo sapiens
Mn2+
-
Homo sapiens
NAD+
-
Homo sapiens
NADH
-
Homo sapiens
NADP+
-
Homo sapiens
NADPH
-
Homo sapiens
terminal 3'-monophosphoryl group of substrates
-
Homo sapiens
UMP
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0006
-
diadenosine 5',5'''-P1,P4-tetraphosphate Ap4A Homo sapiens
0.001
-
diadenosine 5',5'''-P1,P3-triphosphate Ap3A Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation Homo sapiens
Co2+ activation Homo sapiens
divalent cations activation Homo sapiens
Mg2+ activation Homo sapiens
Ni2+ slight activation Homo sapiens
Zn2+ activation Homo sapiens
Zn2+ physiologically bound Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16000
-
x * 66000 + x * 45000 + x * 16000, enzyme has a complex structure, which is composed of subunits of different size, stoichiometry of the complex is still uncertain, SDS-PAGE Homo sapiens
45000
-
x * 66000 + x * 45000 + x * 16000, enzyme has a complex structure, which is composed of subunits of different size, stoichiometry of the complex is still uncertain, SDS-PAGE Homo sapiens
66000
-
x * 66000 + x * 45000 + x * 16000, enzyme has a complex structure, which is composed of subunits of different size, stoichiometry of the complex is still uncertain, SDS-PAGE Homo sapiens
230000
-
gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diadenosine polyphosphate + H2O Homo sapiens phosphodiesterase can be considered as a catabolic enzyme AMP + adenosine polyphosphate-1
-
?
additional information Homo sapiens enzyme might function as a modulating factor of platelet aggregation ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
blood plasma
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine 5'-P1-tetraphospho-P4-5'''-adenosine + H2O
-
Homo sapiens AMP + ATP
-
?
ATP + H2O
-
Homo sapiens 5'-AMP + diphosphate
-
?
diadenosine 5',5'''-P1,P3-triphosphate + H2O
-
Homo sapiens AMP + ADP
-
?
diadenosine polyphosphate + H2O phosphodiesterase can be considered as a catabolic enzyme Homo sapiens AMP + adenosine polyphosphate-1
-
?
dinucleotides + H2O
-
Homo sapiens ?
-
?
additional information no activity with p-nitrophenylthymidine 3'-phosphate Homo sapiens ?
-
?
additional information broad substrate specificity Homo sapiens ?
-
?
additional information enzyme might function as a modulating factor of platelet aggregation Homo sapiens ?
-
?
NAD+ + H2O
-
Homo sapiens 5'-AMP + NMN
-
?
p-nitrophenylthymidine 5'-phosphate + H2O
-
Homo sapiens 5'-TMP + p-nitrophenol
-
?
UTP + H2O
-
Homo sapiens UMP + diphosphate
-
?

Subunits

Subunits Comment Organism
? x * 66000 + x * 45000 + x * 16000, enzyme has a complex structure, which is composed of subunits of different size, stoichiometry of the complex is still uncertain, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
Ap3A (Ap4A) hydrolase
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
Homo sapiens