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Literature summary for 3.1.4.1 extracted from

  • Harvey, C.L.; Olson, K.C.; Wright, R.
    Further purification and properties of phosphodiesterase from the carrot (1970), Biochemistry, 9, 921-925.
    View publication on PubMed
Search for more literature for 3.1.4.1

Application

Application Comment Organism
analysis useful tool in nucleic acid research, characterization of oligonucleotides and as an aid in sequence analysis Daucus carota

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
 Daucus carota
EDTA inactivation reversed by Mg2+, Mn2+, Ca2+, Zn2+ and slightly by Co2+ Daucus carota
glutathione
-
 Daucus carota
L-cysteine
-
 Daucus carota
Reducing agents
-
 Daucus carota
terminal 3'-monophosphoryl group of substrates
-
 Daucus carota

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Daucus carota
Co2+ slight activation Daucus carota
divalent cations required for activity Daucus carota
Mg2+
-
 Daucus carota
Mn2+
-
 Daucus carota
Zn2+
-
 Daucus carota

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
98000 115000 gel filtration Daucus carota

Organism

Organism UniProt Comment Textmining
Daucus carota
-
 carrot
-

Purification (Commentary)

Purification (Comment) Organism
-
 Daucus carota

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Daucus carota
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
253.3
-
-
 Daucus carota

Storage Stability

Storage Stability Organism
-20°C, 0.01 M Tris-HCl, pH 7.6, 0.5 M KCl Daucus carota

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O pyrophosphatase activity Daucus carota AMP + phosphate
-
 ?
ATP + H2O pyrophosphatase activity Daucus carota 5'-AMP + diphosphate
-
 ?
DNA + H2O enzyme hydrolyzes single-stranded DNA, no activity with double-stranded DNA Daucus carota DNA(n-1) + 2'-deoxynucleoside 5'-phosphate
-
 ?
additional information
-
 Daucus carota ?
-
 ?
NAD+ + H2O pyrophosphatase activity Daucus carota 5'-AMP + NMN
-
 ?
oligonucleotides + H2O specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner Daucus carota oligonucleotides(n-1) + 5'-mononucleotides
-
 ?
p-nitrophenylthymidine 5'-phosphate + H2O
-
 Daucus carota 5'-TMP + p-nitrophenol
-
 ?
poly d(A-T)2 + H2O
-
 Daucus carota ?
-
 ?
polynucleotides + H2O specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner Daucus carota polynucleotides(n-1) + 5'-mononucleotides
-
 ?
RNA + H2O
-
 Daucus carota RNA(n-1) + nucleoside 5'-phosphate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 stable at, without loss of activity Daucus carota

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 9.5
-
 Daucus carota