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Literature summary for 3.1.4.1 extracted from

  • Fitt, P.S.; Baddoo, P.
    Separation and purification of the alkaline phosphatase and a phosphodiesterase from Halobacterium cutirubrum (1979), Biochem. J., 181, 347-353.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.1

General Stability

General Stability Organism
(NH4)2SO4 stabilizes Halobacterium salinarum
Mn2+ stabilizes Halobacterium salinarum

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activation Halobacterium salinarum

Organism

Organism UniProt Comment Textmining
Halobacterium salinarum
-
 extremely halophilic bacterium, mixture of 3'- and 5'-exonuclease
-

Purification (Commentary)

Purification (Comment) Organism
partial, mixture of 3'- and 5'-exonuclease Halobacterium salinarum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Halobacterium salinarum

Storage Stability

Storage Stability Organism
4-5°C, dilute solution, loses about 5% of activity per week Halobacterium salinarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bis(p-nitrophenyl) phosphate + H2O
-
 Halobacterium salinarum ?
-
 ?
DNA + H2O
-
 Halobacterium salinarum DNA(n-1) + 2'-deoxynucleoside 5'-phosphate
-
 ?
p-nitrophenylthymidine 5'-phosphate + H2O
-
 Halobacterium salinarum 5'-TMP + p-nitrophenol
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Halobacterium salinarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9
-
 Halobacterium salinarum