Literature summary for 3.1.31.1 extracted from

Allan, J.; Fraser, R.M.; Owen-Hughes, T.; Keszenman-Pereyra, D.
Micrococcal nuclease does not substantially bias nucleosome mapping (2012), J. Mol. Biol., 417, 152-164.

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Application

Application	Comment	Organism
analysis	the enzyme is very widely used to map nucleosomes	Staphylococcus aureus

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure analysis, PDB ID 2SNS	Staphylococcus aureus

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	P00644	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	substrates are chicken and recombinant frog chromatins, as well as mixture of two plasmid DNAs, one harbouring a 10841-bp segment of sheep DNA containing the beta-lactoglobulin gene and the other harbouring a 13626-bp segment of Saccharomyces cerevisiae DNA incorporating a late-firing replication yeast replication origin, reconstituted with limiting amounts of core histones by salt gradient dialysis. Chromatins, prepared by reconstitution with either chicken or frog histones, are digested to mononucleosomes using micrococcal nuclease, identification of the locations and quantification of the strength of both the chicken or frog histone octamer binding sites on each DNA, the enzyme shows sequence specificity in its preferred cleavage sites with a preference to cut at sites centred on A/T-containing dinucleotides, and comparison to the activity of caspase-activated DNase, overview	Staphylococcus aureus	?	-	?

Synonyms

Synonyms	Comment	Organism
MNase	-	Staphylococcus aureus

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Release 2023.1 (January 2023)