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Literature summary for 3.1.30.2 extracted from
- Biochemical properties and hormonal regulation of barley nuclease (1987), Eur. J. Biochem., 168, 357-364.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | - |
Hordeum vulgare |  |
| EDTA | - |
Hordeum vulgare | |
| SDS | - |
Hordeum vulgare | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | no reactivation after EDTA treatment | Hordeum vulgare | |
| additional information | no reactivation after EDTA treatment by Cd2+, Cu2+ | Hordeum vulgare | |
| Zn2+ | reactivates after EDTA treatment | Hordeum vulgare | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
1 * 33000, decarboxylated, SDS-PAGE | Hordeum vulgare |
| 35000 | - |
immunoprecipitation | Hordeum vulgare |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hordeum vulgare | - |
barley | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | about 8% carbohydrate, asparagin-linked high-mannose oligosaccharide | Hordeum vulgare |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Hordeum vulgare | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | - |
Hordeum vulgare | 5'-phosphonucleotides + 5'-phosphomononucleotides | - |
? | |
| additional information | inactive towards 3'-phosphoester linkage of nucleoside cyclic 2',3'- and 3',5'-monophosphates | Hordeum vulgare | ? | - |
? | |
| additional information | enzyme also shows 3'-nucleotidase activity | Hordeum vulgare | ? | - |
? | |
| additional information | 3'-nucleotidase activity is greater for purine than for pyrimidine ribonucleotides | Hordeum vulgare | ? | - |
? | |
| additional information | little activity towards ribonucleoside 2'- and 5'-monophosphates and deoxyribonucleoside 3'- and 5'-monophosphates | Hordeum vulgare | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 33000, decarboxylated, SDS-PAGE | Hordeum vulgare |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| barley nuclease | - |
Hordeum vulgare |
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Release 2023.1 (January 2023)
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