Cloned (Comment) | Organism |
---|---|
gene ENDO1, DNA and amino acid sequence determination and analysis, recombinant transient overexpression in Arabidosis thaliana leaf protoplasts | Arabidopsis thaliana |
gene ENDO2, DNA and amino acid sequence determination and analysis, recombinant transient overexpression in Arabidosis thaliana leaf protoplasts | Arabidopsis thaliana |
gene ENDO4, DNA and amino acid sequence determination and analysis, recombinant transient overexpression in Arabidosis thaliana leaf protoplasts | Arabidopsis thaliana |
gene ENDO5, DNA and amino acid sequence determination and analysis, recombinant transient overexpression in Arabidosis thaliana leaf protoplasts | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | complete inhibition; complete inhibition | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates at pH 8.0 | Arabidopsis thaliana | |
Ca2+ | activates at pH 8.0, preferred divalent cation | Arabidopsis thaliana | |
Mn2+ | activates | Arabidopsis thaliana | |
Mn2+ | activates, highly activating divalent cation | Arabidopsis thaliana | |
additional information | although ENDO1 nuclease digests ssDNA in the presence of both Ca2+ and Mn2+ ions, its ability to digest dsDNA can be stimulated only by Mn2+ ions. The enzyme activity is not affected by Mg2+ or Fe2+ | Arabidopsis thaliana | |
additional information | metal ion effect is dependent on pH. The enzyme activity is not affected by Mg2+ or Fe2+ | Arabidopsis thaliana | |
additional information | the enzyme activity is not affected by Mg2+ or Fe2+ | Arabidopsis thaliana | |
Zn2+ | dependent on | Arabidopsis thaliana | |
Zn2+ | dependent on, Zn2+-dependent activity of the enzyme can be strongly enhanced by the Ca2+ | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arabidopsis thaliana | isozyme ENDO2 digests ssDNA over dsDNA, it achieves strong DNase activity under two completely different conditions, but it exerts its nuclease activity towards dsDNA only in acidic buffer as a Ca2+/Zn2+-dependent enzyme | ? | - |
? | |
additional information | Arabidopsis thaliana | isozyme ENDO4 shows quite strong DNase activity, but relatively weak RNase activity | ? | - |
? | |
additional information | Arabidopsis thaliana | no activity by isozyme ENDO5 on ssDNA or dsDNA | ? | - |
? | |
additional information | Arabidopsis thaliana | the isozyme ENDO1 is defined as bifunctional enzymes due to its ability to digest both DNA and RNA substrates, showing a quite strong DNase activity and weak RNase activity, it digests ssDNA over dsDNA | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | F4JJL0 | gene ENDO4 | - |
Arabidopsis thaliana | F4JJL3 | gene ENDO5 | - |
Arabidopsis thaliana | Q9C9G4 | cf. EC 3.1.30.1; gene ENDO2 | - |
Arabidopsis thaliana | Q9SXA6 | cf. EC 3.1.30.1; gene ENDO1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endosperm | - |
Arabidopsis thaliana | - |
flower | expression of ENDO1 is associated with flower development, expression in stamen, sepal and petal | Arabidopsis thaliana | - |
additional information | in late endosperm the decrease in isozyme ENDO2 is accompanied by a significant increase in isozyme ENDO1 nucleases | Arabidopsis thaliana | - |
petal | - |
Arabidopsis thaliana | - |
seed | - |
Arabidopsis thaliana | - |
seed | high level of ENDO2 transcription is maintained specifically in chalazal endosperm from the preglobular to the linear cotyledon stage and rapidly decreases at later stages of seed development | Arabidopsis thaliana | - |
seedling | - |
Arabidopsis thaliana | - |
sepal | - |
Arabidopsis thaliana | - |
stamen | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | isozyme ENDO2 digests ssDNA over dsDNA, it achieves strong DNase activity under two completely different conditions, but it exerts its nuclease activity towards dsDNA only in acidic buffer as a Ca2+/Zn2+-dependent enzyme | Arabidopsis thaliana | ? | - |
? | |
additional information | isozyme ENDO4 shows quite strong DNase activity, but relatively weak RNase activity | Arabidopsis thaliana | ? | - |
? | |
additional information | no activity by isozyme ENDO5 on ssDNA or dsDNA | Arabidopsis thaliana | ? | - |
? | |
additional information | the isozyme ENDO1 is defined as bifunctional enzymes due to its ability to digest both DNA and RNA substrates, showing a quite strong DNase activity and weak RNase activity, it digests ssDNA over dsDNA | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BFN1 | - |
Arabidopsis thaliana |
ENDO1 | - |
Arabidopsis thaliana |
ENDO2 | - |
Arabidopsis thaliana |
ENDO4 | - |
Arabidopsis thaliana |
ENDO5 | - |
Arabidopsis thaliana |
endonuclease 1 | - |
Arabidopsis thaliana |
endonuclease 2 | - |
Arabidopsis thaliana |
endonuclease 4 | - |
Arabidopsis thaliana |
endonuclease 5 | - |
Arabidopsis thaliana |
nuclease I | - |
Arabidopsis thaliana |
plant S1-like nuclease | - |
Arabidopsis thaliana |
S1-like nuclease | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
optimal activity at acidic pH | Arabidopsis thaliana |
additional information | - |
isozyme ENDO5 is active in the neutral buffer and less efficiently in the acidic buffer | Arabidopsis thaliana |
additional information | - |
optimal activity at neutral pH | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the plant S1-like nucleases class of enzymes. Different members of this family are characterized by a surprisingly large variety of catalytic properties, nucleolytic activities of all Arabidopsis thaliana S1-like paralogues, overview. In addition to Zn2+-dependent enzymes, this family also comprises nucleases activated by Ca2+ and Mn2+, which implies that the apparently well-known S1 nuclease active site in plant nucleases is able to cooperate with different activatory ions. Particular members of this class differ in their optimum pH value and substrate specificity. Plant representatives of this family evolve toward an increase in catalytic diversity. Phylogenetic analysis, overview | Arabidopsis thaliana |
physiological function | involvement of isozyme ENDO1 in endosperm senescence. Plant S1-like nucleases are the main class of enzymes involved in nucleic acid degradation during plant programmed cell death | Arabidopsis thaliana |
physiological function | plant S1-like nucleases are the main class of enzymes involved in nucleic acid degradation during plant programmed cell death | Arabidopsis thaliana |