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Literature summary for 3.1.30.2 extracted from

  • Filimonova, M.; Gubskaya, V.; Nuretdinov, I.
    Some features of hydrolysis of the hybrid B-Z-form dna by Serratia marcescens nuclease (2014), OnLine J. Biol. Sci., 14, 179-185.
No PubMed abstract available
Search for more literature for 3.1.30.2

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ binding of Co(NH3)6 3+ to highly polymerized DNA caused correspondingly about 7fold decrease in Km and more than 600fold decrease in Kcat compared with that of Mg-DNA complex of B-form; binding of Mg2+ to highly polymerized DNA causes correspondingly about 80fold decrease in Km and more than 1600fold decrease in Kcat compared with that of Mg-DNA complex of B-form Serratia marcescens
additional information DNA transition from the right handed B-form to the hybrid B-Z-form causes a decrease in Vmax, Km and Kcat of DNA cleavage with the nuclease Serratia marcescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics of the enzyme with differently structured DNA substrate and different metal ions, overview Serratia marcescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Serratia marcescens the enzyme potently degrades both DNA and RNA and is a nuclease with broad specificity, but it shows some sensitivity to the secondary structure of the substrate ?
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 ?

Organism

Organism UniProt Comment Textmining
Serratia marcescens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
B-form DNA + H2O B form of herring testis DNA Serratia marcescens ?
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 ?
B-Z-hybrid form DNA + H2O hybrid B-Z form of herring testis DNA Serratia marcescens ?
-
 ?
additional information the enzyme potently degrades both DNA and RNA and is a nuclease with broad specificity, but it shows some sensitivity to the secondary structure of the substrate Serratia marcescens ?
-
 ?
additional information substrate is highly polymerized herring testis DNA of the random nucleotide sequence. Hydrolysis of the B-form and hybrid B-Z form DNA by the enzyme. The hybrid B-Z form is formed upon addition of MgSO4 and Co(NH3)6Cl3 Serratia marcescens ?
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 ?

Synonyms

Synonyms Comment Organism
Serratia marcescens nuclease
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 Serratia marcescens
Sma nuc
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 Serratia marcescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Serratia marcescens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.44
-
 B-Z-hybrid form DNA pH 8.0, 30°C, in presence of Mg2+ Serratia marcescens
1.2
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 B-Z-hybrid form DNA pH 8.0, 30°C, in presence of Co(NH3)6 3+ Serratia marcescens
737.6
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 B-form DNA pH 8.0, 30°C, in presence of Mg2+ Serratia marcescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Serratia marcescens