Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 50% inhibition | Thinopyrum elongatum | |
Cu2+ | slight inhibition | Thinopyrum elongatum | |
DTT | slight inhibition | Thinopyrum elongatum | |
Mn2+ | slight inhibition | Thinopyrum elongatum | |
additional information | no inhibition by NEM and iodoacetic acid | Thinopyrum elongatum | |
NaCl | slight inhibition | Thinopyrum elongatum | |
Ni2+ | slight inhibition | Thinopyrum elongatum | |
putrescine | - |
Thinopyrum elongatum | |
spermidine | - |
Thinopyrum elongatum | |
spermine | 70% inhibition at 0.1 mM | Thinopyrum elongatum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect by Mg2+ | Thinopyrum elongatum | |
Zn2+ | stimulates | Thinopyrum elongatum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
1 * 34000, SDS-PAGE | Thinopyrum elongatum |
36000 | - |
gel filtration | Thinopyrum elongatum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dsDNA + H2O | Thinopyrum elongatum | hydrolysis of nucleic acids | ? | - |
? | |
additional information | Thinopyrum elongatum | substrate specificity in descending order is ssDNA, dsDNA, RNA | ? | - |
? | |
RNA + H2O | Thinopyrum elongatum | hydrolysis of nucleic acids | ? | - |
? | |
ssDNA + H2O | Thinopyrum elongatum | hydrolysis of nucleic acids, preference for ssDNA | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thinopyrum elongatum | - |
8x = Elytrigia turcica | - |
Purification (Comment) | Organism |
---|---|
native enzyme 265fold to homogeneity from seedlings by ammonium sulfate fractionation, anion exchange chromatography and heparin affinity chromatography | Thinopyrum elongatum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Thinopyrum elongatum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
purified enzyme | Thinopyrum elongatum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-CCTGGCAGTT + H2O | synthetic labeled deoxydecanucleotide, cleavage of 5'-Ap*G bond and with lower activity of Gp*T, Cp*A, and Gp*G bonds | Thinopyrum elongatum | ? | - |
? | |
dsDNA + H2O | hydrolysis of nucleic acids | Thinopyrum elongatum | ? | - |
? | |
additional information | substrate specificity in descending order is ssDNA, dsDNA, RNA | Thinopyrum elongatum | ? | - |
? | |
additional information | substrate specificity, no activity with dinucleoside monophosphates, overview | Thinopyrum elongatum | ? | - |
? | |
poly(I) + H2O | synthetic homopolyribonucleotide | Thinopyrum elongatum | ? | - |
? | |
polyA + H2O | synthetic homopolyribonucleotide | Thinopyrum elongatum | ? | - |
? | |
polyC + H2O | synthetic homopolyribonucleotide | Thinopyrum elongatum | ? | - |
? | |
polyG + H2O | synthetic homopolyribonucleotide | Thinopyrum elongatum | ? | - |
? | |
polyU + H2O | synthetic homopolyribonucleotide | Thinopyrum elongatum | ? | - |
? | |
RNA + H2O | hydrolysis of nucleic acids | Thinopyrum elongatum | ? | - |
? | |
ssDNA + H2O | hydrolysis of nucleic acids, preference for ssDNA | Thinopyrum elongatum | ? | - |
? | |
supercoiled plasmid DNA + H2O | - |
Thinopyrum elongatum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 34000, SDS-PAGE | Thinopyrum elongatum |
Synonyms | Comment | Organism |
---|---|---|
endonuclease | - |
Thinopyrum elongatum |
nuclease A | - |
Thinopyrum elongatum |
plant type I nuclease | - |
Thinopyrum elongatum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Thinopyrum elongatum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
10 min, loss of 30% of activity | Thinopyrum elongatum |
70 | - |
10 min, loss of 90% of activity | Thinopyrum elongatum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Thinopyrum elongatum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thinopyrum elongatum | isoelectric focusing | - |
4.7 |