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Literature summary for 3.1.30.2 extracted from
- Action of hexaamminecobalt on the activity of Serratia marcescens nuclease (2003), BioMetals, 16, 447-453.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 744 | - |
Mg2+ | pH 8.5, 25ºC | Serratia marcescens |  |
| 2898 | - |
[Co(NH3)6]3+ | pH 8.5, 25ºC | Serratia marcescens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Serratia marcescens | |
| [Co(NH3)6]3+ | binding to the DNA substrate induces changes in the secondary structure of the enzyme, followed by a decrease of the enzyme activity | Serratia marcescens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Serratia marcescens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ion exchange chromatography on DEAE-cellulose DE-32 and DEAE-cellulose DE-52 | Serratia marcescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| highly polymerized DNA + H2O | - |
Serratia marcescens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Sm2 | isoform, mature Sma nuc | Serratia marcescens |
| Sma nuc | - |
Serratia marcescens |
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Release 2023.1 (January 2023)
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