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Literature summary for 3.1.3.9 extracted from

  • Ghosh, A.; Shieh, J.J.; Pan, C.J.; Sun, M.S.; Chou, J.Y.
    The Catalytic Center of Glucose-6-phosphatase (2002), J. Biol. Chem., 277, 32837-32842.
    View publication on PubMed
Search for more literature for 3.1.3.9

Application

Application Comment Organism
medicine deficiency in enzyme causes glycogen storage disease type 1, and s large number of enzyme mutations is identified in glycogen storage disease type 1a patients Homo sapiens

Protein Variants

Protein Variants Comment Organism
H119A mutation inactivates the phosphatase Homo sapiens
H176A mutation inactivates the phosphatase Homo sapiens
H179A mutation completely abolishes the enzyme activity Homo sapiens
H197T no significant change of enzyme activity Homo sapiens
H252A no significant change of enzyme activity Homo sapiens
H307A no significant change of enzyme activity Homo sapiens
H353A no significant change of enzyme activity Homo sapiens
H52A no significant change of enzyme activity Homo sapiens
H9A no significant change of enzyme activity Homo sapiens
K76N mutation inactivates the phosphatase Homo sapiens
R170Q mutation inactivates the phosphatase Homo sapiens
R83C mutation inactivates the phosphatase Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum the enzyme is anchored by nine transmembrane helices Homo sapiens 5783
-
microsome
-
 Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 Western blot analysis Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose 6-phosphate + H2O Homo sapiens key enzyme in D-glucose homeostasis D-glucose + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
the phosphate-enzyme intermediate Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
D-glucose 6-phosphate + H2O = D-glucose + phosphate reaction mechanism, His176 is the amino acid in enzyme that acts as the nucleophile forming the phosphohistidine-enzyme intermediate during catalysis Homo sapiens
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.102
-
 activity using pSVL expression system Homo sapiens
1.268
-
 activity using adenoviral expression system Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose 6-phosphate + H2O
-
 Homo sapiens D-glucose + phosphate
-
 ?
D-glucose 6-phosphate + H2O key enzyme in D-glucose homeostasis Homo sapiens D-glucose + phosphate
-
 ?