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Literature summary for 3.1.3.9 extracted from
- The glucose-6-phosphatase system (2002), Biochem. J., 362, 513-532.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-glucose | elevated concentration, D-glucose may play a direct role as a stimulator of enzyme expression | Homo sapiens |  |
| dexamethasone | in cultured hepatoma cells | Rattus norvegicus | |
| glycocorticoids | injection of glycocorticoids in vivo increases to a modestextent the activity of enzyme in the livers of control and adrenalectomized rats | Rattus norvegicus |
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | the enzyme deficiency is responsible for glycogen storage disease type I, starvation and diabetes cause a 2-3fold increase in enzyme activity in the liver | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Mus musculus |
- |
Rattus norvegicus |
| cloning of two principal constituents: the catalytic subunit and D-glucose 6-phosphate translocase, expression studies in COS cells | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-Mercaptopicolinic acid | inhibitor of D-glucose 6-phosphate translocase | Homo sapiens | |
| 4,4'-di-isothiocyanostilbene-2,2'-disulphonate | inhibitor of D-glucose 6-phosphate translocase | Homo sapiens | |
| chlorogenic acid | inhibitor of D-glucose 6-phosphate translocase | Homo sapiens | |
| D-glucose | non-competitive inhibitor, irrespective of the presence of detergents | Homo sapiens | |
| Insulin | insulin causes a decrease in the activity of enzyme in the liver in vivo | Homo sapiens | |
| kodaistatin A | A and C | Homo sapiens | |
| additional information | The enzyme is inhibited by several amphiphilic compounds, such as fatty acids and acyl-CoAs, but the physioligal significance is questionable, since the liver contains a fatty-acyl CoA binding protein, which may well prevent this effect. Some thiol reagents inhibit enzyme activity much more in intact than in disrupted microsomes. | Homo sapiens | |
| mumbaistatin | - |
Homo sapiens | |
| phosphate | non-competitive inhibition in intact microsomes, but competitive in the presence of detergents | Homo sapiens | |
| tosyl-lysyl-chloromethane | inhibitor of D-glucose 6-phosphate translocase | Homo sapiens | |
| tosylphenylalanylchloromethane | inhibitor of D-glucose 6-phosphate translocase | Homo sapiens | |
| tungstate | - |
Homo sapiens | |
| vanadate | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | 3 | D-glucose 6-phosphate | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | lumen | Homo sapiens | 5783 | - |
| membrane | membrane topology: a model with six transmembrane domains are first propsed, but has been superseded by a model with nine transmembrane domains | Homo sapiens | 16020 | - |
| microsome | - |
Homo sapiens | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate + H2O | Homo sapiens | the enzyme plays a important role of providing D-glucose during starvation | D-glucose + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| The treatment with detergents, wheter anionic, cationic or neutral, modestly stimulates the hydrolysis of D-glucose 6-phosphate, but considerably increases the phosphatase activity on other substrates, such as mannose 6-phosphate | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| D-glucose 6-phosphate + H2O = D-glucose + phosphate | reaction mechanism: substrate-transport model | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| astrocyte | low enzyme activities | Homo sapiens | - |
| hepatoma cell | - |
Rattus norvegicus | - |
| kidney | cortex | Mus musculus | - |
| kidney | cortex | Homo sapiens | - |
| kidney | cortex, both mRNA and activity of enzyme appear during the first days of extratrauterine life, reaching a maximum after 2-3 weeks | Rattus norvegicus | - |
| liver | - |
Mus musculus | - |
| liver | - |
Homo sapiens | - |
| liver | enzyme activities greatly increases in rat liver around birth, the activities appears to be less specific in extracts of fetal liver enzyme than of adult liver | Rattus norvegicus | - |
| additional information | the enzyme is expressed in beta-cells of pancreatic islets and in intestinal musoca, most particulary in the starved and diabetic states | Mus musculus | - |
| additional information | the enzyme is expressed in beta-cells of pancreatic islets and in intestinal musoca, most particulary in the starved and diabetic states | Homo sapiens | - |
| additional information | the enzyme is expressed in beta-cells of pancreatic islets and in intestinal musoca, most particulary in the starved and diabetic states | Rattus norvegicus | - |
| placenta | syncytiotrophoblasts | Homo sapiens | - |
| skeletal muscle | low enzyme activities | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate + H2O | - |
Mus musculus | D-glucose + phosphate | - |
? | |
| D-glucose 6-phosphate + H2O | - |
Homo sapiens | D-glucose + phosphate | - |
? | |
| D-glucose 6-phosphate + H2O | - |
Rattus norvegicus | D-glucose + phosphate | - |
? | |
| D-glucose 6-phosphate + H2O | the enzyme plays a important role of providing D-glucose during starvation | Homo sapiens | D-glucose + phosphate | - |
? | |
| D-mannose 6-phosphate + H2O | - |
Homo sapiens | D-mannose + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | a 50000 Da protein is identified as D-glucose 6-phosphate translocase | Rattus norvegicus |
| More | a 55000 Da protein is identified as D-glucose 6-phosphate translocase | Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | 0.007 | tungstate | in detergent treated microsomes | Homo sapiens | |
| 0.0022 | - |
vanadate | in detergent treated microsomes | Homo sapiens | |
| 0.0056 | - |
vanadate | in intact microsomes | Homo sapiens | |
| 0.01 | 0.025 | tungstate | in intact microsomes | Homo sapiens | |
| 0.035 | - |
4,4'-di-isothiocyanostilbene-2,2'-disulphonate | - |
Homo sapiens | |
| 0.5 | - |
chlorogenic acid | - |
Homo sapiens | |
| 50 | 200 | D-glucose | - |
Homo sapiens | |
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